| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSSK2 |
| Uniprot No | Q96PF2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMDDATVL RKKGYIVGIN LGKGSYAKVK SAYSERLKFN VAVKIIDRKK TPTDFVERFL PREMDILATV NHGSIIKTYE IFETSDGRIY IIMELGVQGD LLEFIKCQGA LHEDVARKMF RQLSSAVKYC HDLDIVHRDL KCENLLLDKD FNIKLSDFGF SKRCLRDSNG RIILSKTFCG SAAYAAPEVL QSIPYQPKVY DIWSLGVILY IMVCGSMPYD DSDIRKMLRI QKEHRVDFPR SKNLTCECKD LIYRMLQPDV SQRLHIDEIL SHSWLQPPKP KATSSASFKR EGEGKYRAEC KLDTKTGLRP DHRPDHKLGA KTQHRLLVVP ENENRMEDRL AETSRAKDHH ISGAEVGKAS T |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSSK2重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Expression and functional characterization of recombinant TSSK2 in mammalian cells"*
**作者**：Zhang Y, et al.
**摘要**：本研究成功构建了哺乳动物细胞中表达的重组TSSK2蛋白，通过亲和层析纯化获得高纯度蛋白。实验验证了其激酶活性，发现TSSK2对特定底物(如H3组蛋白)的磷酸化能力，并探讨了其在精子发生中调控染色质浓缩的作用。
2. **文献名称**：*"Structural insights into TSSK2 kinase using recombinant protein crystallography"*
**作者**：Li H, et al.
**摘要**：利用重组TSSK2蛋白进行晶体结构解析，揭示了其ATP结合域和催化中心的构象特征。研究为开发靶向TSSK2的小分子抑制剂(如男性避孕药物)提供了结构基础，并验证了重组蛋白在体外酶活实验中的应用潜力。
3. **文献名称**：*"TSSK2 recombinant protein as a biomarker for male infertility screening"*
**作者**：Wang X, et al.
**摘要**：通过分析不育男性精液样本，发现TSSK2蛋白表达水平与精子活力显著相关。研究团队开发了基于重组TSSK2的抗体制备技术，建立ELISA检测方法，提出其作为男性不育诊断生物标志物的可能性。
*注*：上述文献为示例性内容，实际研究中建议通过PubMed或Web of Science以关键词"TSSK2 recombinant"检索最新论文。
**Background of TSSK2 Recombinant Protein**
TSSK2 (Testis-Specific Serine/Threonine Kinase 2) is a member of the serine/threonine kinase family predominantly expressed in the testis, specifically in post-meiotic spermatids during late spermatogenesis. It plays a critical role in sperm maturation and function, particularly in processes such as chromatin condensation, acrosome formation, and sperm motility. TSSK2 is essential for male fertility, as evidenced by studies in knockout mice, which exhibit sterility due to defective sperm maturation and structural abnormalities.
The recombinant TSSK2 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cells, to produce a purified, biologically active form of the kinase. This recombinant protein retains the enzymatic activity of native TSSK2. enabling researchers to study its phosphorylation substrates, regulatory mechanisms, and interactions with other proteins (e.g., TSKS, a substrate involved in sperm head shaping). Structural studies reveal that TSSK2 contains a conserved kinase domain critical for its catalytic function, along with unique N- and C-terminal regions that may regulate its localization or activity.
Research on TSSK2 has significant implications for understanding male infertility and developing contraceptives. Its testis-specific expression and indispensable role in spermatogenesis make it a potential target for non-hormonal male contraceptives. Inhibitors targeting TSSK2’s kinase activity could disrupt sperm maturation without affecting systemic hormone levels. Additionally, mutations or dysregulation of TSSK2 have been linked to human infertility, underscoring its clinical relevance.
The availability of recombinant TSSK2 protein facilitates *in vitro* assays, drug screening, and antibody production, advancing both basic research and therapeutic development. Ongoing studies aim to elucidate its signaling pathways and validate its utility in diagnostic or therapeutic applications.
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