首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠可溶性E选择素(sE-selectin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SELE含量。SELE即选择素E，是内皮细胞在炎性因子刺激下表达的黏附分子。其背景围绕炎症反应，在多种炎症相关疾病中表达异常。研究机制表明，它能介导白细胞与内皮细胞的黏附、滚动，参与炎症细胞募集，在炎症、肿瘤转移等病理过程意义重大。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于心血管疾病、糖尿病并发症等炎症相关动物模型的机制研究，或体外细胞实验中内皮细胞激活程度的动态监测。为研究炎症信号通路、药物干预效果或组织修复机制提供可靠工具，满足基础科研中对靶标蛋白定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
缩写：SELE
Uniprot No.：P98105
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 88-108 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 89-100 | |||||
| 1:40 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 93-105 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.7462.6452.6962.54225001.9761.8751.9261.77212501.2281.2041.2161.0626250.7820.7610.7720.6183120.5220.5110.5170.3631560.3630.3520.3580.204780.2900.2850.2880.13400.1540.1530.154 | ||||||
货期：3-5 working days
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