| WB | 咨询技术 | Transfected |
| IF | 咨询技术 | Transfected |
| IHC | 咨询技术 | Transfected |
| ICC | 技术咨询 | Transfected |
| FCM | 咨询技术 | Transfected |
| Elisa | 咨询技术 | Transfected |
| Aliases | Proto-oncogene Wnt-3; INT4; TETAMS |
| Entrez GeneID | 7473 |
| clone | 7H11 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Transfected |
| Immunogen | Purified recombinant human Wnt3 protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Wnt3抗体的3篇示例参考文献(内容为模拟概括，非真实文献，仅供参考)：
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1. **文献名称**：*Monoclonal Antibody Targeting Wnt3 Inhibits Tumor Growth in Colorectal Cancer Models*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种特异性靶向Wnt3蛋白的单克隆抗体，通过体外和体内实验证明其能有效抑制结直肠癌细胞中Wnt/β-catenin信号通路的活性，显著降低肿瘤增殖并诱导细胞凋亡。
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2. **文献名称**：*Wnt3 Antibody-Based Detection of Protein Localization in Embryonic Development*
**作者**：Chen L, et al.
**摘要**：利用Wnt3特异性多克隆抗体，研究者在小鼠胚胎发育模型中揭示了Wnt3蛋白在神经管和体节形成中的时空表达模式，证实其在早期形态发生中的关键作用。
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3. **文献名称**：*A Novel Wnt3 Neutralizing Antibody Attenuates Osteoarthritis Progression*
**作者**：Wang Y, et al.
**摘要**：该研究设计了一种人源化Wnt3中和抗体，通过阻断Wnt3与受体结合，显著减轻骨关节炎模型中的软骨退化和炎症反应，为临床治疗提供了潜在策略。
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**注意**：以上为示例性内容，真实文献需通过PubMed、Google Scholar等数据库检索，建议结合关键词“Wnt3 antibody”和“应用场景(如癌症、发育等)”筛选近期或高引研究。
**Background of Wnt3 Antibody**
The Wnt3 antibody is a specialized tool used to detect and study Wnt3. a member of the Wnt family of secreted glycoproteins critical in developmental signaling pathways. Wnt3 plays a key role in the Wnt/β-catenin-dependent (canonical) and -independent (non-canonical) pathways, regulating processes like embryonic development, cell proliferation, differentiation, and tissue homeostasis. Dysregulation of Wnt3 is implicated in cancers, congenital disorders, and degenerative diseases.
Wnt3 antibodies are designed to specifically bind to Wnt3 proteins, enabling researchers to visualize their expression, localization, and interactions in various biological samples. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. They often target conserved regions of Wnt3. such as its C-terminal or N-terminal domains, ensuring specificity.
Monoclonal Wnt3 antibodies offer high reproducibility, while polyclonal versions may detect broader epitopes. Their applications include studying Wnt3's role in embryogenesis, stem cell regulation, and cancer progression (e.g., colorectal or breast cancer). Additionally, Wnt3 antibodies aid in exploring therapeutic targets by identifying aberrant Wnt3 signaling in disease models. Validation of these antibodies involves testing for cross-reactivity with other Wnt family members to ensure accuracy. Overall, Wnt3 antibodies are indispensable for dissecting the molecular mechanisms of Wnt3 in health and disease.
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