| WB | 咨询技术 | Transfected |
| IF | 咨询技术 | Transfected |
| IHC | 咨询技术 | Transfected |
| ICC | 技术咨询 | Transfected |
| FCM | 咨询技术 | Transfected |
| Elisa | 咨询技术 | Transfected |
| Aliases | Wnt5b |
| Entrez GeneID | 81029 |
| clone | 3G1 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 45 kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Transfected |
| Immunogen | Purified recombinant human Wnt5b protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Wnt5b抗体的3篇代表性文献示例(注：部分信息为模拟概括，实际文献需根据具体数据库核实)：
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1. **文献名称**: "Wnt5b regulates epithelial-mesenchymal transition in melanoma via AKT/GSK-3β signaling"
**作者**: Smith A, et al.
**摘要**: 本研究利用Wnt5b特异性抗体，通过免疫组织化学和Western blot分析，发现Wnt5b在黑色素瘤中高表达，并通过AKT/GSK-3β通路促进肿瘤细胞侵袭和转移。抗体特异性通过siRNA敲低实验验证。
2. **文献名称**: "Development and validation of a polyclonal antibody against human Wnt5b for colorectal cancer studies"
**作者**: Chen L, et al.
**摘要**: 文章报道了一种兔源多克隆抗体的制备流程，该抗体针对人Wnt5b蛋白C端结构域。通过ELISA、免疫荧光和流式细胞术验证其高亲和力和特异性，并应用于结直肠癌组织中Wnt5b表达与预后的相关性分析。
3. **文献名称**: "Wnt5b-mediated non-canonical signaling drives obesity-associated adipose tissue inflammation"
**作者**: Tanaka K, et al.
**摘要**: 研究使用Wnt5b中和抗体在小鼠模型中发现，Wnt5b通过激活JNK/STAT3通路加剧脂肪组织炎症和胰岛素抵抗，提示其作为代谢性疾病的潜在治疗靶点。
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**注意**：以上为模拟文献，实际引用请通过PubMed、Google Scholar等平台以“Wnt5b antibody”或“Wnt5b function”为关键词检索近年研究。
Wnt5b antibodies are immunological tools designed to detect and study Wnt5b, a member of the Wnt protein family critical in developmental processes and disease pathways. Wnt5b, a secreted glycoprotein, acts as a ligand in both canonical (β-catenin-dependent) and non-canonical signaling pathways. It regulates cell proliferation, differentiation, migration, and tissue patterning during embryogenesis and adult tissue homeostasis. Dysregulation of Wnt5b is implicated in cancers (e.g., breast, gastric, colorectal), metabolic disorders, and skeletal abnormalities, making it a key research target.
These antibodies, often monoclonal or polyclonal, are generated against specific epitopes of Wnt5b and validated for applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and ELISA. They enable researchers to visualize Wnt5b expression, localization, and interaction with receptors like Frizzled and ROR1/2. High-quality Wnt5b antibodies exhibit specificity, minimal cross-reactivity with homologous proteins (e.g., Wnt5a), and robust performance across experimental models, including cell lines, tissues, and animal samples. Recent studies utilize these antibodies to explore Wnt5b’s role in tumor metastasis, stem cell regulation, and therapeutic resistance, highlighting their value in mechanistic and translational research. Validations using knockout controls or functional assays (e.g., ligand neutralization) are essential to confirm antibody reliability. As Wnt signaling gains attention in drug development, Wnt5b antibodies remain pivotal for uncovering its pathophysiological roles and therapeutic potential.
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