首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠多配体聚糖4(SDC4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SDC4含量。SDC4是一个值得关注的靶点，目前其具体背景与研究机制介绍缺失。一般而言，靶点研究通常围绕其在细胞内的信号传导、与疾病发生发展的关联等方面开展，后续需进一步探索SDC4在特定生理和病理过程中的具体作用机制。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于低丰度靶标的高精度分析；在肿瘤微环境、炎症反应及代谢性疾病等基础研究中具有重要价值；可支持体外培养模型、动物实验样本的蛋白表达水平检测；为探索SDC4在细胞行为调控、疾病发生发展中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sdc4; Syndecan-4; SYND4; Ryudocan core protein
缩写：SDC4
Uniprot No.：O35988
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SDC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:200 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:400 | Average % | 94 | |||||
| Range % | 90-100 | ||||||
| 1:800 | Average % | 106 | |||||
| Range % | 102-113 | ||||||
回收率：
| The recovery of mouse SDC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-95 | |||||
| EDTA plasma (n=4) | 97 | 92-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected2002.7042.6592.6822.5341002.2182.2712.2452.097501.5761.6541.6151.467250.9971.0121.0050.85712.50.6110.5930.6020.4546.250.4080.4250.4170.2693.120.2740.2680.2710.12300.1460.1500.148 | |||||||
货期：3-5 working days
×
