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产品描述：大鼠Syndecan-1/CD138(SDC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SDC1含量。SDC1是一个靶点。它是一种细胞表面蛋白聚糖，在多种生理和病理过程中发挥作用。研究显示，其参与细胞增殖、黏附、迁移等机制。在肿瘤领域，SDC1表达异常与肿瘤发生、发展及转移密切相关，成为肿瘤治疗等方面的研究热点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品可广泛应用于大鼠模型研究中SDC1表达的动态监测，例如探究炎症性疾病发展机制、肿瘤微环境中细胞间相互作用，或评估组织损伤修复过程中SDC1的调控功能，为分子机理研究及药物干预效果评价提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sdc1 ELISA Kit; Synd1 ELISA Kit; Syndecan-1 ELISA Kit; SYND1 ELISA Kit; CD antigen CD138 ELISA Kit
缩写：SDC1
Uniprot No.：P26260
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 88-102 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 83-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of rat SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 94-107 | ||||
| EDTA plasma (n=4) | 100 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.4582.4222.4402.264 102.0041.9761.9901.814 51.3071.3451.3261.150 2.50.7280.7370.7330.557 1.250.4320.4480.4400.264 0.6250.3330.3420.3380.162 0.3120.2510.2430.2470.071 00.1740.1780.176 | ||||||
货期：3-5 working days
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