| WB | 咨询技术 | Transfected |
| IF | 咨询技术 | Transfected |
| IHC | 咨询技术 | Transfected |
| ICC | 技术咨询 | Transfected |
| FCM | 咨询技术 | Transfected |
| Elisa | 咨询技术 | Transfected |
| Aliases | 4EBP2; PHASII |
| Entrez GeneID | 1979 |
| clone | 9C3 |
| WB Predicted band size | Calculated MW: 13 kDa; Observed MW: 15-20 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Transfected |
| Immunogen | Recombinant protein of human 4E-BP2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3条关于4E-BP2抗体的参考文献示例(内容为模拟概括，仅供参考)：
1. **文献名称**：*Regulation of 4E-BP2 phosphorylation and degradation by mTOR signaling*
**作者**：Gingras AC et al.
**摘要**：研究阐明了mTORC1通过磷酸化4E-BP2调控其与eIF4E结合的分子机制，使用特异性抗体检测了不同磷酸化状态下的4E-BP2蛋白表达，揭示其在翻译起始中的动态调控。
2. **文献名称**：*4E-BP2 knockout mice exhibit impaired hippocampal synaptic plasticity*
**作者**：Banko JL et al.
**摘要**：通过4E-BP2特异性抗体进行脑组织免疫组化分析，发现4E-BP2缺失导致小鼠海马神经元突触可塑性异常，提示其在学习记忆中的关键作用。
3. **文献名称**：*Antibody-based profiling of 4E-BP2 isoforms in human cancers*
**作者**：Tcherkezian J et al.
**摘要**：开发了一种高特异性4E-BP2抗体，用于Western blot和免疫荧光分析，验证了4E-BP2在不同癌症组织中的异构体表达差异及其与肿瘤进展的关联。
注：以上为模拟生成的示例，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“4E-BP2 antibody”、“4E-BP2 phosphorylation”查找具体研究。
The 4E-BP2 antibody is a crucial tool in studying the eukaryotic initiation factor 4E (eIF4E)-binding protein family, particularly 4E-BP2 (eIF4E-binding protein 2). 4E-BP2. along with 4E-BP1 and 4E-BP3. regulates cap-dependent translation by binding to eIF4E, a key component of the translation initiation complex. This interaction inhibits eIF4E from assembling the eIF4F complex, thereby suppressing mRNA translation. The phosphorylation status of 4E-BP2. modulated by mTORC1 signaling, determines its binding affinity to eIF4E. Dysregulation of this pathway is implicated in cancers, neurodegenerative diseases, and metabolic disorders.
The 4E-BP2 antibody enables researchers to detect and quantify endogenous 4E-BP2 protein levels, assess its phosphorylation state, and study its role in cellular processes like proliferation, autophagy, and stress responses. Its specificity is critical due to high sequence homology among 4E-BP isoforms. Studies using this antibody have revealed tissue-specific expression patterns, with 4E-BP2 being abundant in the brain, suggesting unique roles in neuronal function. Recent work highlights its involvement in cancer resistance mechanisms when 4E-BP1 is inhibited, making it a potential therapeutic target. The antibody is widely applied in Western blotting, immunoprecipitation, and immunofluorescence, advancing our understanding of translational control in health and disease.
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