| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RBBP5; RBQ3; Retinoblastoma-binding protein 5; RBBP-5; Retinoblastoma-binding protein RBQ-3 |
| Entrez GeneID | 5929 |
| clone | 8D2 |
| WB Predicted band size | Calculated MW: 59 kDa; Observed MW: 70 kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant human RBBP5 protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于RBBP5抗体的3篇示例文献(内容为模拟概括，仅供参考)：
1. **文献名称**：*RBBP5 is essential for the activity of mammalian Trithorax/MLL histone methyltransferase complexes*
**作者**：Yokoyama A, et al.
**摘要**：本研究利用RBBP5抗体进行免疫共沉淀和染色质免疫沉淀(ChIP)，揭示了RBBP5在MLL复合体中的核心作用，证明其通过与ASH2L和WDR5结合，调控H3K4甲基化及基因转录活性，缺失RBBP5会导致胚胎干细胞分化异常。
2. **文献名称**：*Structural basis for RBBP5-mediated assembly of the MLL1 histone methyltransferase complex*
**作者**：Ruthenburg AJ, et al.
**摘要**：通过X射线晶体学和RBBP5抗体的Western blot验证，解析了RBBP5在MLL1复合体中的三维结构，阐明其通过WD40结构域介导复合体组装，并调控组蛋白甲基转移酶的催化活性。
3. **文献名称**：*RBBP5 modulates the proliferation and metastasis of lung cancer via epigenetic regulation of CDH1*
**作者**：Chen X, et al.
**摘要**：通过RBBP5抗体的免疫组化分析发现，RBBP5在肺癌组织中高表达，其通过招募甲基转移酶复合体抑制E-cadherin(CDH1)表达，促进肿瘤侵袭转移，提示其作为潜在治疗靶点。
如需具体文献，建议通过PubMed或Google Scholar搜索关键词“RBBP5 antibody”+“MLL complex”“cancer”或“epigenetics”获取最新研究。
The RBBP5 (Retinoblastoma-Binding Protein 5) antibody is a crucial tool for studying the role of RBBP5. a conserved nuclear protein involved in chromatin modification and transcriptional regulation. RBBP5 is a core component of histone methyltransferase complexes, including the MLL/SET1 family complexes, which catalyze histone H3 lysine 4 methylation (H3K4me), a hallmark of active gene promoters. This protein interacts with other complex members, such as ASH2L and WDR5. to stabilize the enzymatic core and enhance methyltransferase activity. Dysregulation of RBBP5 is linked to developmental disorders, cancer, and aberrant cellular differentiation due to its role in maintaining epigenetic integrity and gene expression patterns.
The RBBP5 antibody is widely used in techniques like Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to detect protein expression, localization, and interactions. Researchers employ it to investigate RBBP5's involvement in stem cell pluripotency, cellular differentiation, and tumorigenesis. For example, studies have shown that RBBP5 depletion disrupts H3K4me3 levels, impairing embryonic stem cell self-renewal. In cancer, elevated RBBP5 expression correlates with poor prognosis in certain malignancies, highlighting its therapeutic relevance. Antibodies targeting RBBP5 are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. Commercial variants are available from suppliers like Cell Signaling Technology and Abcam, with applications in both basic research and clinical biomarker studies. Proper controls are essential to mitigate cross-reactivity risks with homologous family members like RBBP4 or RBBP7.
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