| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPAN7 |
| Uniprot No | P41732 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 113-213aa |
| 氨基酸序列 | ADPRHEIKDT FLRTYTDAMQ TYNGNDERSR AVDHVQRSLS CCGVQNYTNW STSPYFLEHG IPPSCCMNET DCNPQDLHNL TVAATKVNQK GCYDLVTSFM ETNMHHHHHH |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于TSPAN7重组蛋白的模拟参考文献(基于公开研究主题概括，非真实文献，仅供参考)：
1. **文献名称**: "TSPAN7重组蛋白在肺癌细胞迁移中的功能研究"
**作者**: Smith A, et al.
**摘要**: 通过基因重组技术表达TSPAN7蛋白，发现其通过调控整合素信号通路抑制肺癌细胞迁移，为靶向治疗提供潜在靶点。
2. **文献名称**: "TSPAN7重组蛋白的结构解析及其与X-linked智力障碍的关联"
**作者**: Chen L, et al.
**摘要**: 利用X射线晶体学解析TSPAN7重组蛋白的跨膜结构域，揭示其突变导致神经元突触功能异常，与X-linked智力障碍密切相关。
3. **文献名称**: "重组TSPAN7蛋白的制备及其在神经再生中的应用"
**作者**: Wang Y, et al.
**摘要**: 优化大肠杆菌表达系统纯化TSPAN7重组蛋白，体外实验证明其促进神经轴突生长，可能用于神经损伤修复。
**注意**：以上文献为示例性内容，实际研究需查阅PubMed、Web of Science等数据库获取真实文献。
TSPAN7 (Tetraspanin 7) is a member of the tetraspanin family of transmembrane proteins, characterized by four conserved transmembrane domains that mediate interactions with other proteins to form functional signaling complexes. These proteins play critical roles in cellular processes such as adhesion, migration, proliferation, and membrane organization. TSPAN7. specifically, is highly expressed in the brain, particularly in the hippocampus, and is implicated in neuronal development, synaptic plasticity, and intracellular signaling pathways. Its association with X-linked intellectual disability (XLID) underscores its importance in cognitive function, as mutations or dysregulation of TSPAN7 have been linked to impaired dendritic spine formation and neural connectivity.
Recombinant TSPAN7 protein is engineered using expression systems like mammalian cells or bacteria to produce purified, biologically active forms of the protein. This engineered protein typically retains functional domains necessary for studying its interactions with partners such as integrins, receptors, or cytoskeletal components. Researchers utilize recombinant TSPAN7 to investigate its role in cellular networks, particularly in neurological disorders and cancer, where altered tetraspanin activity is often observed. For instance, studies explore how TSPAN7 modulates pathways like PI3K/AKT or influences metalloproteinase activity in tumor microenvironments. Additionally, it serves as a tool for developing diagnostic markers or therapeutic targets, given its cell-surface accessibility and disease correlations. The availability of recombinant TSPAN7 enables detailed structural analysis, antibody development, and high-throughput screening for drug discovery, bridging gaps in understanding its physiological and pathological mechanisms.
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