| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACG; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKA CAT around the phosphorylation site of Thr197. AA range:166-215 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-PKA alpha/beta/gamma (Thr197)抗体的3篇参考文献示例：
1. **文献名称**：*"cAMP-dependent protein kinase: structure, regulation, and role in disease"*
**作者**：Taylor, S.S. et al.
**摘要**：该综述详细阐述了PKA的结构与功能，重点分析了Thr197位点的磷酸化对其催化亚基激活的关键作用，并提及使用Phospho-PKA (Thr197)抗体验证其在细胞信号转导中的活性变化。
2. **文献名称**：*"AKAP-mediated compartmentalization of PKA signaling in cancer progression"*
**作者**：Zhang, Y. et al.
**摘要**：研究利用Phospho-PKA (Thr197)抗体通过免疫印迹和免疫荧光技术，揭示PKA在肿瘤细胞中因AKAP蛋白锚定导致的异常磷酸化及空间调控机制，及其与肿瘤转移的关联。
3. **文献名称**：*"Phosphorylation of PKA catalytic subunit in cardiac hypertrophy"*
**作者**：Müller, C.W. et al.
**摘要**：通过心肌细胞模型，研究证实压力超负荷诱导的PKA Thr197位点磷酸化水平升高与心脏肥厚相关，使用特异性抗体检测到该位点的动态变化，提示其作为病理标志物的潜力。
(注：上述文献为示例性内容，实际引用需根据具体研究补充真实文献信息。)
The Phospho-PKA alpha/beta/gamma (Thr197) antibody detects endogenous protein kinase A (PKA) catalytic subunits (α, β, or γ) when phosphorylated at threonine 197. a critical post-translational modification for PKA activation. PKA is a serine/threonine kinase central to cAMP-dependent signaling, regulating processes like metabolism, gene expression, and cell proliferation. It consists of two regulatory subunits that inhibit catalytic subunits under low cAMP levels. Upon cAMP elevation, regulatory subunits dissociate, releasing active catalytic subunits.
Thr197 phosphorylation occurs in the catalytic subunit's activation loop, stabilizing the active conformation by facilitating proper alignment of the kinase domain. This autophosphorylation (or mediated by upstream kinases) is essential for full enzymatic activity. The antibody specifically recognizes this phosphorylation event across PKA α (PRKACA), β (PRKACB), and γ (PRKACG) isoforms, enabling researchers to study PKA activation dynamics in diverse tissues.
Applications include Western blotting, immunohistochemistry, and immunofluorescence to assess PKA activity in physiological or pathological contexts, such as metabolic disorders, cardiovascular diseases, or cancer. Its specificity makes it valuable for investigating cAMP-PKA signaling aberrations linked to endocrine tumors, neurodegenerative conditions, or drug responses. Proper controls (e.g., phosphatase-treated samples) are recommended to confirm phosphorylation-dependent signals.
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