| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TF; Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin |
| Entrez GeneID | 7018 |
| clone | 2H8 |
| WB Predicted band size | Calculated MW: 77 kDa; Observed MW: 77 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant protein expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与转铁蛋白抗体相关的文献示例(注：文献标题和作者为虚拟示例，供参考)：
1. **《Transferrin receptor-targeted antibody-drug conjugates in cancer therapy》**
- 作者：Daniels, T.R., et al.
- 摘要：研究利用转铁蛋白受体抗体开发靶向药物偶联物(ADCs)，通过抗体特异性结合肿瘤细胞表面过表达的转铁蛋白受体，增强化疗药物的靶向递送，减少系统性毒性。
2. **《Development of a high-sensitivity ELISA for human serum transferrin using monoclonal antibodies》**
- 作者：Smith, J.K., et al.
- 摘要：报道了一种基于单克隆抗体的高灵敏度ELISA检测方法，用于定量人血清中转铁蛋白水平，验证了其在贫血和铁代谢疾病诊断中的临床应用价值。
3. **《Antibody-mediated inhibition of transferrin receptor 1 reduces amyloid-β pathology in a mouse model of Alzheimer’s disease》**
- 作者：Wang, Y., et al.
- 摘要：研究通过抗转铁蛋白受体1抗体阻断脑内皮细胞的转铁蛋白摄取通路，减少β淀粉样蛋白在大脑中的沉积，为阿尔茨海默病的治疗提供新策略。
注：以上文献信息为模拟内容，实际引用需通过PubMed、Web of Science等数据库检索真实文献。
Transferrin antibodies are immunoglobulins designed to target transferrin, a glycoprotein critical for iron transport in blood plasma. Transferrin binds and delivers ferric ions (Fe³⁺) to cells via receptor-mediated endocytosis, maintaining iron homeostasis and preventing oxidative damage from free iron. Its role in cellular iron uptake makes it a biomarker in conditions like iron-deficiency anemia, inflammation, and certain cancers.
Antibodies against transferrin are widely used in research and diagnostics. In immunoassays (e.g., ELISA, Western blot), they enable quantification of transferrin levels in serum or tissue samples, aiding in diagnosing hypochromic anemias or monitoring nutritional status. Immunohistochemistry applications leverage these antibodies to localize transferrin expression in cells, providing insights into iron metabolism disorders or tumor microenvironments.
Transferrin antibodies are categorized as polyclonal or monoclonal. Polyclonal antibodies recognize multiple epitopes, enhancing detection sensitivity, while monoclonal antibodies offer high specificity for single epitopes, reducing cross-reactivity. Both types are essential in developing diagnostic kits and experimental tools. Additionally, transferrin antibodies serve as loading controls in protein analysis, ensuring equal sample loading in techniques like Western blotting.
Recent advances include recombinant antibodies with improved consistency and engineered fragments for specialized applications. Their utility spans clinical diagnostics, therapeutic development, and mechanistic studies of iron-related pathologies.
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