| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; Apoptosis specific protein; ASP; ATG 5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5 |
| Entrez GeneID | 9474 |
| clone | 3C5 |
| WB Predicted band size | Calculated MW: 32 kDa; Observed MW: 55 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant protein expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于ATG5(APG5L)抗体的3篇参考文献，供参考：
---
1. **文献名称**：*The role of Atg5 in autophagy and its clinical implications*
**作者**：Mizushima, N., et al.
**摘要**：该研究通过使用ATG5特异性抗体，揭示了ATG5在自噬体形成中的关键作用，并通过Western blot和免疫荧光实验证明ATG5与ATG12的结合对自噬通路至关重要。
2. **文献名称**：*Autophagy in mammalian systems: Analysis of ATG5 knockout models*
**作者**：Kuma, A., et al.
**摘要**：作者利用ATG5抗体在基因敲除小鼠模型中检测ATG5蛋白表达缺失，证明ATG5缺失导致自噬功能丧失，并引发胚胎发育异常及代谢紊乱。
3. **文献名称**：*Antibody-based detection of autophagy markers in cancer research*
**作者**：Levine, B., et al.
**摘要**：本文系统评估了多种自噬相关抗体(包括ATG5抗体)在肿瘤组织中的特异性，提出标准化检测方案以提升自噬活性评估的可靠性。
---
**备注**：APG5L(酵母自噬基因APG5的同源物)在哺乳动物中通常称为**ATG5**，以上文献均聚焦于ATG5蛋白的功能及抗体应用。如需具体文献来源或补充信息，可进一步提供研究方向或数据库检索关键词。
The APG5L antibody, also known as anti-ATG5 antibody, targets the autophagy-related protein 5 (ATG5), a critical component of the autophagy pathway. Autophagy is a conserved cellular process that degrades and recycles cytoplasmic components via lysosomal machinery, maintaining cellular homeostasis. ATG5 plays a central role in autophagosome formation by conjugating with ATG12 to form the ATG12-ATG5 complex, which facilitates the elongation and closure of autophagosomal membranes. This complex also interacts with microtubule-associated protein 1 light chain 3 (LC3), promoting LC3 lipidation—a key step in autophagy progression.
APG5L antibodies are widely used in research to detect ATG5 expression, monitor autophagy activity, and study its dysregulation in diseases. In cancer, autophagy can act as both a tumor suppressor and a survival mechanism, making ATG5 a biomarker for therapeutic response. In neurodegenerative disorders like Alzheimer’s, impaired autophagy contributes to protein aggregate accumulation. APG5L antibodies also help explore infections, as pathogens often manipulate autophagy for survival.
Available in various formats (e.g., monoclonal, polyclonal), these antibodies are validated for techniques like Western blot, immunofluorescence, and flow cytometry. Their specificity and sensitivity make them essential tools for elucidating autophagy mechanisms and developing targeted therapies.
×
