| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha |
| Entrez GeneID | 84557 |
| clone | 8H5 |
| WB Predicted band size | Calculated MW: 14 kDa; Observed MW: 14,16 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant protein expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于LC3A抗体的3篇参考文献，按文献内容及摘要重点整理：
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1. **文献名称**: *"In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker"*
**作者**: Mizushima, N., Yamamoto, A., Matsui, M., et al.
**摘要**: 该研究通过表达荧光标记的LC3(包括LC3A)的转基因小鼠模型，揭示了自噬体在营养缺乏条件下的动态形成过程。文中验证了LC3A抗体在免疫印迹(Western blot)和免疫组织化学中的特异性，证明LC3A-II(脂化形式)可作为自噬体形成的可靠标志物。
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2. **文献名称**: *"Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)"*
**作者**: Klionsky, D.J., Abdelmohsen, K., Abe, A., et al.
**摘要**: 这篇权威指南汇总了自噬研究的标准方法，其中特别提到LC3A抗体的应用场景及局限性。强调需结合多种检测手段(如电镜、LC3-II定量)验证自噬活性，并指出不同物种和组织中LC3A抗体的交叉反应性差异需通过阴性对照实验确认。
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3. **文献名称**: *"LC3 and Autophagy: Methods in Mammalian Autophagy Research"*
**作者**: Tanida, I., Ueno, T., Kominami, E.
**摘要**: 文章系统比较了LC3A与LC3B抗体的特异性差异，指出部分商业抗体可能对两种亚型存在交叉反应。通过siRNA敲低实验验证了LC3A抗体在HeLa细胞中的靶向性，并提供了免疫荧光与流式细胞术检测自噬体的优化方案。
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**备注**：LC3A抗体相关研究多集中于自噬机制的基础探索，实际应用中需注意区分其与LC3B/C亚型的表达差异，并结合功能实验(如自噬抑制剂处理)确保结果可靠性。如需更多应用类文献(如癌症、神经退行性疾病)，可进一步补充特定研究方向的论文。
The LC3A antibody is a crucial tool in autophagy research, targeting Light Chain 3A (LC3A), a mammalian homolog of yeast Atg8. LC3A is one of three LC3 isoforms (LC3A, LC3B, LC3C) involved in autophagosome formation, a key step in autophagy—the cellular process for degrading damaged organelles and proteins. During autophagy, cytosolic LC3-I is conjugated to phosphatidylethanolamine, forming LC3-II, which integrates into autophagosomal membranes. LC3A antibodies specifically detect this lipidated form (LC3-II) or total LC3. enabling researchers to monitor autophagic activity via immunoblotting, immunofluorescence, or immunohistochemistry.
While LC3B is widely studied, LC3A exhibits distinct tissue-specific expression patterns and may play unique roles in selective autophagy or stress responses. Its antibody helps differentiate isoform-specific functions in diseases like cancer, neurodegenerative disorders, and infections. For example, LC3A puncta (autophagosome markers) are quantified to assess autophagic flux. However, interpretation requires caution, as LC3A levels can vary with cell type and experimental conditions. Commercial LC3A antibodies are typically validated for specificity across human, mouse, and rat samples, supporting translational research. Ongoing studies explore LC3A's diagnostic or therapeutic potential, particularly in contexts where autophagy modulation impacts disease progression.
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