| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | beta I tubulin; TBB1; TUBB1; Tubulin beta 1; Class VI beta tubulin |
| Entrez GeneID | 81027 |
| clone | 7H5 |
| WB Predicted band size | Calculated MW: 50 kDa; Observed MW: 50 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of β I tubulin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于βI Tubulin抗体的3篇文献摘要示例：
1. **文献名称**：*"βI Tubulin as a Marker for Neuronal Differentiation"*
**作者**：Smith J. et al.
**摘要**：研究利用βI Tubulin抗体分析神经元分化过程中微管动态变化，证实其在神经元轴突特异性标记中的应用，并揭示其与微管稳定性的关联。
2. **文献名称**：*"Immunohistochemical Detection of βI Tubulin in Cancer Tissues"*
**作者**：Lee M. et al.
**摘要**：通过βI Tubulin抗体进行免疫组化分析，发现乳腺癌组织中βI Tubulin高表达与化疗耐药性显著相关，提示其作为预后标志物的潜力。
3. **文献名称**：*"Development and Validation of a βI Tubulin-Specific Monoclonal Antibody"*
**作者**：Patel R. et al.
**摘要**：报道一种高特异性βI Tubulin单克隆抗体的开发，验证其在Western blot和免疫荧光中的灵敏度和特异性，并对比其与其他Tubulin亚型的交叉反应性。
4. **文献名称**：*"βI Tubulin in Cellular Mitosis and Disease Pathogenesis"*
**作者**：Johnson K. et al.
**摘要**：综述βI Tubulin在细胞有丝分裂中的功能，总结其在神经退行性疾病及癌症中的作用，并讨论相关抗体在基础研究与临床诊断中的应用。
(注：上述文献为示例，实际引用时需查询真实文献。)
Beta I tubulin (βI-tubulin), a member of the tubulin protein family, is a critical component of microtubules—dynamic cytoskeletal structures essential for cell division, intracellular transport, and maintenance of cell shape. As one of multiple β-tubulin isotypes, βI-tubulin (encoded by the *TUBB* gene in humans) is ubiquitously expressed across tissues and plays a foundational role in microtubule assembly and function. Its conserved structure includes GTP-binding domains and regions critical for polymerization into protofilaments, which form the microtubule lattice.
Antibodies targeting βI-tubulin are widely used in research to study microtubule dynamics, cell cycle progression, and cytoskeletal organization. These antibodies enable visualization and quantification of microtubules in techniques like immunofluorescence, immunohistochemistry, and Western blotting. Due to βI-tubulin’s constitutive expression, it is also employed as a loading control in protein analyses, though cross-reactivity with other β-tubulin isoforms (e.g., βIII-tubulin in neurons) requires careful validation of antibody specificity.
In disease research, βI-tubulin antibodies help investigate roles in cancer (e.g., resistance to microtubule-targeting chemotherapies), neurodevelopmental disorders, and ciliopathies. Commercial βI-tubulin antibodies are often raised against conserved epitopes, such as the C-terminal region, and are available from major suppliers (e.g., Sigma-Aldrich, Abcam). Their reliability and broad applicability make them indispensable tools in cell biology and biomedical research.
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