| WB | 咨询技术 | Rabbit |
| IF | 咨询技术 | Rabbit |
| IHC | 咨询技术 | Rabbit |
| ICC | 技术咨询 | Rabbit |
| FCM | 咨询技术 | Rabbit |
| Elisa | 咨询技术 | Rabbit |
| Aliases | Antibody; Ig gamma chain C region; Ig kappa-b4 chain C region; Ig kappa-b5 chain C region; Ig kappa-b9 chain C region; Ig lambda chain C region; K-BAS. |
| Entrez GeneID | - |
| clone | 7H9 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rabbit |
| Immunogen | Recombinant Protein of Rabbit IgG (Light Chain Specific) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇与Rabbit IgG Light Chain抗体相关的文献摘要示例(注：文献信息为模拟内容，实际引用请核实数据库)：
1. **文献名称**: *"Production and characterization of rabbit monoclonal antibodies specific for IgG light chains"*
**作者**: Smith J, et al.
**摘要**: 该研究描述了通过杂交瘤技术制备兔源单克隆抗体，特异性识别IgG轻链(κ和λ型)。抗体经ELISA和Western blot验证，适用于人及小鼠样本的免疫沉淀实验，且在石蜡包埋组织中显示良好免疫组化性能。
2. **文献名称**: *"Cross-reactivity analysis of anti-rabbit IgG light chain antibodies in multispecies applications"*
**作者**: Lee H, et al.
**摘要**: 文章评估了商品化兔IgG轻链抗体与不同物种(如小鼠、大鼠、犬)IgG的交叉反应性，发现部分抗体可通过非还原电泳特异性识别轻链，提示其在跨物种研究中的潜在应用价值，但需优化封闭条件以减少背景信号。
3. **文献名称**: *"Optimization of light chain-specific antibodies for quantitative flow cytometry in rabbit models"*
**作者**: Garcia R, et al.
**摘要**: 研究团队开发了一种针对兔IgG轻链的荧光标记抗体，通过流式细胞术实现B细胞表面IgG的定量检测。该抗体在兔自身免疫疾病模型中验证了其高灵敏度和低非特异性结合特性。
建议通过PubMed或Google Scholar以关键词 **"rabbit IgG light chain antibody" + application** 检索最新文献，优先选择近5年发表的、经同行评审的实验性论文或方法学文章。
Rabbit IgG light chain antibodies are integral components of immunoglobulin G (IgG) molecules, which play a central role in adaptive immune responses. IgG antibodies consist of two identical heavy chains and two identical light chains linked by disulfide bonds. The light chain, with a molecular weight of approximately 25 kDa, is composed of a variable (VL) and a constant (CL) domain. In rabbits, most IgG light chains are of the kappa (κ) type, with lambda (λ) chains being rare (<5%), unlike in humans where both types are common.
Light chains contribute to antigen-binding specificity through their variable regions, which form part of the antibody's paratope. The constant region stabilizes the antibody structure and facilitates interactions with other immune components. Anti-rabbit IgG light chain antibodies are widely used as detection tools in techniques like Western blotting, ELISA, and immunohistochemistry. They are particularly valuable for identifying rabbit-derived primary antibodies in multiplex assays without cross-reacting with heavy chains or antibodies from other species, ensuring specificity.
These antibodies are often produced in hosts like goats or mice immunized with purified rabbit light chain fragments. Advances in recombinant antibody technology have enabled the development of monoclonal anti-light chain antibodies with enhanced consistency and reduced batch variability. Their applications extend to immune monitoring, autoimmune disease research, and quality control in therapeutic antibody production. Proper validation remains critical to avoid cross-reactivity with non-target proteins or light chains from other species.
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