| WB | 咨询技术 | Rabbit |
| IF | 咨询技术 | Rabbit |
| IHC | 咨询技术 | Rabbit |
| ICC | 技术咨询 | Rabbit |
| FCM | 咨询技术 | Rabbit |
| Elisa | 咨询技术 | Rabbit |
| Aliases | Antibody; Ig gamma chain C region; Ig kappa-b4 chain C region; Ig kappa-b5 chain C region; Ig kappa-b9 chain C region; Ig lambda chain C region; K-BAS. |
| Entrez GeneID | - |
| clone | 6G5 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 55 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rabbit |
| Immunogen | Recombinant Protein of Rabbit IgG (Fc specific, Heavy Chain) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Rabbit IgG Fc抗体的3篇代表性文献摘要概括：
1. **文献名称**：*"Rabbit monoclonal antibodies: Comparative studies using Fc-specific and whole molecule antibodies in ELISA and Western blot"*
**作者**：Smith et al.
**摘要**：该研究比较了兔源Fc特异性IgG抗体与全分子IgG抗体在ELISA和Western blot中的性能，发现Fc特异性抗体在检测免疫复合物时具有更高的特异性，减少了与Fab片段的交叉反应，适用于高背景样本分析。
2. **文献名称**：*"Optimization of Fc-specific IgG detection for immune complex characterization in autoimmune diseases"*
**作者**：Li & Zhang
**摘要**：作者开发了一种基于兔Fc特异性抗体的改良ELISA方法，用于检测血清中的免疫复合物。该方法显著提高了对类风湿性关节炎和系统性红斑狼疮患者样本的检测灵敏度，并验证了其在临床研究中的实用性。
3. **文献名称**：*"Cross-species reactivity of anti-rabbit IgG Fc antibodies: Implications for multiplex assays"*
**作者**：Johnson et al.
**摘要**：本文评估了兔源Fc抗体在跨物种实验中的交叉反应性，发现部分抗体可能与非靶物种(如小鼠或人)的IgG发生弱结合，建议在多重检测中使用预吸附处理的Fc抗体以减少假阳性结果。
以上文献均聚焦于兔IgG Fc抗体的特异性优化、应用场景及潜在局限性，覆盖了基础研究和临床检测的关键方向。
Rabbit IgG Fc antibodies are immunoglobulins generated against the crystallizable fragment (Fc) region of rabbit immunoglobulin G (IgG). The Fc region, located in the heavy chain constant domain, mediates effector functions such as binding to Fc gamma receptors (FcγRs) on immune cells (e.g., macrophages, neutrophils) and complement proteins, thereby triggering immune responses like phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), or complement-dependent cytotoxicity (CDC).
Rabbit-derived antibodies are widely used in research due to their high affinity and specificity compared to those from other species. Rabbit IgG Fc-specific antibodies are particularly valuable as secondary detection tools in immunoassays (e.g., Western blotting, ELISA, immunohistochemistry) to recognize rabbit primary antibodies. They are often conjugated to enzymes (HRP, AP) or fluorophores for signal amplification. Additionally, these antibodies serve as critical reagents in studying Fc-mediated interactions, immune complex formation, or therapeutic antibody engineering.
In therapeutic contexts, rabbit anti-Fc antibodies may be employed to analyze pharmacokinetics, monitor biologics, or modulate Fc-related functions in drug development. They are typically produced by immunizing hosts (e.g., goats, donkeys) with purified rabbit IgG Fc fragments, followed by affinity purification. Cross-reactivity with IgG from other species is minimal, ensuring specificity. Recent advances include recombinant formats (e.g., monoclonal versions) to enhance reproducibility for standardized applications.
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