| WB | 咨询技术 | Rabbit |
| IF | 咨询技术 | Rabbit |
| IHC | 咨询技术 | Rabbit |
| ICC | 技术咨询 | Rabbit |
| FCM | 咨询技术 | Rabbit |
| Elisa | 咨询技术 | Rabbit |
| Aliases | Antibody; Ig gamma chain C region; Ig kappa-b4 chain C region; Ig kappa-b5 chain C region; Ig kappa-b9 chain C region; Ig lambda chain C region; K-BAS. |
| Entrez GeneID | - |
| clone | 5G2 |
| WB Predicted band size | Calculated MW: 25,55 kDa; Observed MW: 25,55 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rabbit |
| Immunogen | Recombinant Protein of Rabbit IgG (H+L) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Rabbit IgG H&L抗体的3篇代表性文献摘要归纳(注：文献为虚构示例，仅用于说明格式)：
1. **文献名称**：*Production and Characterization of Rabbit IgG (H&L) Antibodies for Immunoassays*
**作者**：Smith A, et al.
**摘要**：研究报道了兔源IgG H&L多克隆抗体的高效制备方法，通过重组蛋白免疫技术获得高亲和力抗体，并验证其在ELISA和Western Blot中的广谱结合能力，适用于检测多种物种来源的一抗。
2. **文献名称**：*Cross-Reactivity Analysis of Rabbit Anti-IgG H&L Antibodies in Multispecies Applications*
**作者**：Johnson R, et al.
**摘要**：系统评估了兔IgG H&L抗体在小鼠、人、大鼠等物种IgG的交叉反应性，证明其通过轻链保守区域实现泛物种识别，为多重免疫检测实验提供了通用二抗选择依据。
3. **文献名称**：*Optimizing Signal Detection: Rabbit IgG F(ab')₂ Fragments in Immunohistochemistry*
**作者**：Lee C, et al.
**摘要**：对比完整IgG与F(ab')₂片段在组织染色中的表现，发现后者通过去除Fc片段显著降低非特异性背景，同时保留H&L区域对抗原的高灵敏度结合特性。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词如"Rabbit IgG H&L antibody characterization"或结合具体应用场景筛选。
Rabbit IgG H&L (Heavy & Light chain) antibodies are secondary antibodies specifically designed to target both the heavy and light chains of rabbit immunoglobulin G (IgG). IgG, a major class of immunoglobulins, consists of two heavy chains (∼50 kDa each) and two light chains (∼25 kDa each) linked by disulfide bonds. The heavy chain contains one variable domain (VH) and three constant domains (CH1. CH2. CH3), while the light chain has one variable (VL) and one constant (CL) domain. H&L antibodies recognize conserved regions across both chains, enabling broad detection of intact rabbit IgG molecules.
These antibodies are widely used as secondary reagents in immunoassays like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They bind to the Fc region of primary rabbit IgG antibodies, often conjugated to enzymes (e.g., HRP, AP) or fluorophores for signal amplification. Produced typically in hosts like donkey or goat, they are affinity-purified to ensure specificity. As polyclonal antibodies, they exhibit high sensitivity due to recognition of multiple epitopes, though cross-reactivity with immunoglobulins from other species must be precluded via adsorption steps during manufacturing.
A key advantage of H&L antibodies is their compatibility with diverse experimental setups, as they detect whole IgG molecules regardless of antigen specificity. However, users must verify host species compatibility to avoid cross-reactivity (e.g., anti-rabbit secondary antibodies should not react with mouse-derived samples). Quality control includes validation against reduced/non-reduced IgG to confirm dual-chain recognition. Their versatility and reliability make them essential tools in research and diagnostic workflows involving rabbit primary antibodies.
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