| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSFM |
| Uniprot No | P43897-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 46-346aa |
| 氨基酸序列 | MSKELLMKLRRKTGYSFVNCKKALETCGGDLKQAEIWLHKEAQKEGWSKA AKLQGRKTKEGLIGLLQEGNTTVLVEVNCETDFVSRNLKFQLLVQQVALG TMMHCQTLKDQPSAYSKVQWLTPVNLALWEAEAGGSLEGFLNSSELSGLP AGPDREGSLKDQLALAIGKLGENMILKRAAWVKVPSGFYVGSYVHGAMQS PSLHKLVLGKYGALVICETSEQKTNLEDVGRRLGQHVVGMAPLSVGSLDD EPGGEAETKMLSQPYLLDPSITLGQYVQPQGVSVVDFVRFECGEGEEAAE TE |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSFM(假设为某种重组蛋白或相关技术)的模拟参考文献示例(注：TSFM的具体定义可能因研究领域而异，以下内容为假设性示例，建议通过学术数据库检索真实文献)：
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1. **文献名称**: "High-yield expression and purification of recombinant TSFM protein in Escherichia coli"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了一种在大肠杆菌系统中高效表达TSFM重组蛋白的优化方法，通过密码子优化和融合标签策略提升可溶性表达，并利用亲和层析技术实现高纯度纯化，为后续功能研究奠定基础。
2. **文献名称**: "Structural and functional characterization of TSFM as a mitochondrial translation factor"
**作者**: Smith J, Patel R.
**摘要**: 通过X射线晶体学解析TSFM蛋白的三维结构，揭示其在线粒体翻译延伸过程中的作用机制，并利用重组TSFM验证其与核糖体及tRNA的相互作用，阐明其功能关键区域。
3. **文献名称**: "TSFM-based chimeric antigen receptor (CAR) T cells for targeted cancer therapy"
**作者**: Wang Y, et al.
**摘要**: 开发了一种基于TSFM重组蛋白的CAR-T细胞疗法，通过将TSFM胞外域与信号传导结构域融合，增强了对特定肿瘤抗原的识别能力，并在小鼠模型中验证了其抗肿瘤效果。
4. **文献名称**: "Role of recombinant TSFM in modulating inflammatory responses in vitro"
**作者**: Kim S, et al.
**摘要**: 探讨重组TSFM蛋白对巨噬细胞炎症反应的调控作用，发现其通过抑制NF-κB信号通路减少促炎因子释放，提示其在炎症性疾病治疗中的潜在应用。
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**注意**：以上文献为示例性质，具体研究需参考真实发表的论文。建议通过PubMed、Google Scholar等平台以“TSFM recombinant protein”或结合具体研究背景(如“mitochondrial translation factor”)为关键词检索相关文献。
**Background of TSFM Recombinant Protein**
TSFM (mitochondrial translation elongation factor Ts) is a nuclear-encoded protein critical for mitochondrial translation machinery. It functions as a guanine nucleotide exchange factor (GEF) for mitochondrial elongation factor Tu (EF-Tu), facilitating GTP regeneration during protein synthesis in mitochondria. This interaction is essential for the elongation phase of mitochondrial translation, ensuring proper assembly of oxidative phosphorylation (OXPHOS) complexes I, III, IV, and V, which are vital for ATP production.
The recombinant TSFM protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems. Recombinant technology enables large-scale production with high purity, overcoming limitations of isolating native TSFM from tissues, which is labor-intensive and yields low quantities. The recombinant form retains functional activity, allowing researchers to study its role in mitochondrial diseases, such as encephalopathies, cardiomyopathies, and Leigh syndrome, linked to TSFM mutations.
Studies utilize TSFM recombinant protein to investigate mitochondrial translation defects, model diseases *in vitro*, and screen therapeutic compounds. Its applications extend to structural biology (e.g., crystallography) to elucidate mechanisms of EF-Tu/TSFM interactions. Additionally, recombinant TSFM serves as a tool to explore metabolic disorders, aging, and cancer, where mitochondrial dysfunction is implicated.
Overall, TSFM recombinant protein bridges molecular research and clinical insights, offering a controlled, reproducible resource to decode mitochondrial biology and develop targeted therapies.
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