首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠S100B蛋白(S-100B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100B含量。S100B是一种钙结合蛋白，在神经系统中广泛分布，尤其在星形胶质细胞内高度表达。其研究机制主要聚焦于它作为神经损伤和神经炎症的标志物，可反映中枢神经系统的损伤程度，也参与细胞增殖、分化、凋亡等过程，在神经系统疾病研究中备受关注。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于神经科学基础研究，如脑损伤动物模型评估、神经保护药物开发或神经系统疾病机制探索，也可用于体外实验中S100B蛋白表达水平的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：S100b ELISA Kit; Protein S100-B ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 calcium-binding protein B ELISA Kit
缩写：S100B
Uniprot No.：P04631
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-93 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-101 | |||||
回收率：
| The recovery of rat S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 89 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 2001.9541.8741.9141.751 1001.5621.5311.5471.384 501.1741.1681.1711.008 250.8020.8160.8090.646 12.50.5670.5520.5600.397 6.250.3470.3550.3510.188 3.120.2640.2610.2630.100 00.1580.1670.163 | ||||||
货期：3-5 working days
×
