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产品描述：兔子S100B蛋白(S-100B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100B含量。S100B是一种神经胶质细胞源性钙结合蛋白，在神经系统中发挥多种作用。其与神经发育、损伤修复等过程密切相关。研究机制主要聚焦于其参与神经细胞内信号传导、调节神经细胞的存活和凋亡等方面，为神经相关疾病的研究提供了重要靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，支持科研人员高效分析不同样本中S100B的浓度动态，适用于神经退行性疾病模型研究、脑损伤动物实验、神经胶质瘤机制探索等基础研究领域。为实验室开展神经生物学、细胞应激反应等研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：S100B ELISA Kit; Protein S100-B ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 calcium-binding protein B ELISA Kit
缩写：S100B
Uniprot No.：Q6YNR6
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 90-99 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 99-110 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of rabbit S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 97 | 92-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.5052.6522.5792.48010001.9681.9761.9721.8735001.2741.2041.2391.1402500.7130.7390.7260.6271250.4360.4240.4300.33162.50.2510.2590.2550.15631.250.1820.1850.1840.08500.0970.1010.099 | |||||||
货期：3-5 working days
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