| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Trypsin-3 |
| Uniprot No | P09943 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | VLLDGNGEVVQNGGTYYLLPQVWAQGGGVQLAKTGEETCPLTVVQSPNEL SDGKPIRIESRLRSAFIPDDDKVRIGFAYAPKCAPSPWWTVVEDEQEGLS VKLSEDESTQFDYPFKFEQVSDQLHSYKLLYCEGKHEKCASIGINRDQKG YRRLVVTEDYPLTVVLKKDESS |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组Trypsin-3(PRSS3)的3篇代表性文献(模拟虚构内容，供参考)：
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1. **文献名称**: *Expression and Functional Characterization of Recombinant Human Trypsin-3 in Insect Cells*
**作者**: Smith A, et al.
**摘要**: 本研究通过杆状病毒-昆虫细胞系统成功表达重组人Trypsin-3.并验证其酶活性在pH 8.0条件下对荧光底物的高效水解能力，为蛋白酶功能研究提供工具。
2. **文献名称**: *PRSS3 Promotes Pancreatic Cancer Metastasis via Activating EGFR Signaling Pathway*
**作者**: Zhang L, et al.
**摘要**: 发现重组Trypsin-3通过切割细胞外基质蛋白并激活EGFR通路，增强胰腺癌细胞侵袭性，提示其作为肿瘤治疗潜在靶点。
3. **文献名称**: *Optimization of Recombinant Trypsin-3 Production in E. coli for Industrial Applications*
**作者**: Kim H, et al.
**摘要**: 通过优化大肠杆菌表达条件(诱导温度、IPTG浓度)，将重组Trypsin-3产量提高3倍，并证明其在生物制药下游加工中的稳定性。
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**备注**：实际文献需通过PubMed/Google Scholar检索关键词"recombinant PRSS3"或"Trypsin-3 expression"获取。可补充具体研究方向(如疾病机制或工业应用)进一步筛选文献。
Trypsin-3. also known as PRSS3. is a serine protease belonging to the trypsin family. It is encoded by the PRSS3 gene in humans and shares structural homology with other trypsin isoforms, including Trypsin-1 and Trypsin-2. Naturally produced in the pancreas, Trypsin-3 plays a role in digestive processes by cleaving peptide bonds at specific amino acid residues (lysine and arginine). Beyond digestion, it is implicated in cellular signaling, tissue remodeling, and inflammatory responses. Dysregulation of Trypsin-3 has been linked to pathologies such as pancreatitis, cancer progression, and inflammatory disorders, highlighting its dual role in physiology and disease.
Recombinant Trypsin-3 is engineered using genetic cloning techniques, typically expressed in microbial (e.g., *E. coli*) or mammalian cell systems to ensure proper folding and enzymatic activity. This recombinant form offers advantages over animal-derived trypsin, including batch-to-batch consistency, reduced contamination risks, and scalability for industrial applications. It retains the catalytic triad (His-57. Asp-102. Ser-195) critical for proteolytic function and is often produced as a proenzyme (zymogen) requiring activation, mimicking its natural regulation.
Research applications of recombinant Trypsin-3 span protein digestion for mass spectrometry, cell dissociation in bioprocessing, and studies exploring protease-activated receptor (PAR) signaling pathways. Its role in cancer metastasis, particularly in activating matrix metalloproteinases (MMPs) or degrading extracellular matrix components, has spurred interest in therapeutic targeting. However, its precise biological functions and regulatory mechanisms remain under investigation, with ongoing studies focusing on isoform-specific activities and inhibitor development. Recombinant Trypsin-3 thus serves as a vital tool for both basic research and biotechnological applications.
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