首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠S100-A1蛋白(S100A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100A1含量。S100A1属于S100蛋白家族成员，主要分布于心肌和骨骼肌等组织。它参与细胞内钙信号调节，可调控心肌收缩与舒张。研究发现其异常表达与心肌病等疾病密切相关，有望成为治疗心血管疾病等的潜在靶点，为相关疾病诊治带来新思路。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于基础科研中探索S100A1在心血管疾病、肌肉功能调控或代谢性疾病模型中的分子机制，可定量分析不同实验条件下（如药物干预、基因编辑或病理刺激）生物样本中S100A1的动态变化，为相关领域的蛋白功能研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：S100a1 ELISA Kit; Protein S100-A1 ELISA Kit; S-100 protein alpha chain ELISA Kit; S-100 protein subunit alpha ELISA Kit; S100 calcium-binding protein A1 ELISA Kit
缩写：S100A1
Uniprot No.：P35467
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 95-107 | ||||||
回收率：
| The recovery of rat S100A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected4002.0432.0012.0221.8512001.2951.3271.3111.1401000.8880.8520.8700.699500.6080.6400.6240.453250.4300.4600.4450.27412.50.3460.3290.3380.1676.250.2550.2380.2470.07600.1680.1740.171 | |||||||
货期：3-5 working days
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