| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRPT1 |
| Uniprot No | Q86TN4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-253aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRPT1重组蛋白的3篇示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Structural Insights into the Catalytic Mechanism of Human TRPT1 tRNA Phosphotransferase*
**作者**：Zhang Y, et al.
**摘要**：本研究通过重组表达人源TRPT1蛋白，利用X射线晶体学解析了其三维结构，揭示了其催化核心的关键氨基酸残基，并证明其在tRNA 3'端修复中的磷酸转移酶活性。
2. **文献名称**：*Functional Characterization of Recombinant TRPT1 in tRNA Splicing and Stress Response*
**作者**：Kim H, Lee M.
**摘要**：作者在大肠杆菌中表达并纯化了重组TRPT1蛋白，通过体外实验证实其参与tRNA剪接后的修复过程，并发现其缺失会导致细胞在氧化应激条件下的生长缺陷。
3. **文献名称**：*Development of a High-Yield Expression System for TRPT1 and Its Biochemical Analysis*
**作者**：Wang X, et al.
**摘要**：该研究优化了TRPT1在昆虫细胞中的重组表达条件，获得高纯度蛋白，并验证其酶活动力学参数，为后续药物筛选提供实验基础。
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**建议**：如需具体文献，可访问PubMed或Google Scholar，以关键词“TRPT1 recombinant protein”“TRPT1 structure/function”检索近年研究。
**Background of TRPT1 Recombinant Protein**
TRPT1 (tRNA 2'-O-ribosylphosphate transferase 1) is an enzyme encoded by the *TRPT1* gene, which plays a role in the post-transcriptional modification of transfer RNA (tRNA). Specifically, TRPT1 catalyzes the transfer of a ribosylphosphate group to the 2'-oxygen of specific nucleotides in tRNA, a modification critical for tRNA stability, structural integrity, and accurate translation. This enzyme is conserved across eukaryotes and prokaryotes, underscoring its fundamental biological importance.
Structurally, TRPT1 belongs to the Rossmann-fold methyltransferase superfamily but uniquely combines methyltransferase-like features with distinct catalytic residues for ribosylphosphate transfer. Its recombinant form is typically produced using expression systems like *E. coli* or mammalian cell cultures, enabling studies on its enzymatic mechanisms and interactions. Recombinant TRPT1 is often tagged (e.g., His-tag) for purification and functional assays.
Research on TRPT1 has revealed connections to cellular stress responses, particularly oxidative stress, as tRNA modifications are sensitive to redox imbalances. Dysregulation of TRPT1 activity may contribute to translational errors or impaired stress adaptation, with potential implications in neurodegenerative diseases and cancer. Additionally, TRPT1 has been linked to DNA repair pathways, suggesting a broader role in maintaining genomic stability.
The availability of recombinant TRPT1 has advanced structural studies (e.g., X-ray crystallography) and high-throughput screening for inhibitors or modulators. These efforts aim to elucidate its role in disease and explore therapeutic strategies targeting tRNA modification pathways. Further research is needed to fully unravel its biological and pathological significance.
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