| WB | 咨询技术 | Human,Mouse,Rat,Pig |
| IF | 咨询技术 | Human,Mouse,Rat,Pig |
| IHC | 1/50-1/100 | Human,Mouse,Rat,Pig |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Pig |
| FCM | 咨询技术 | Human,Mouse,Rat,Pig |
| Elisa | 1/10000 | Human,Mouse,Rat,Pig |
| Aliases | PRKAA1; AMPK1; 5' -AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK |
| Entrez GeneID | 5562/5563 |
| WB Predicted band size | Calculated MW: 64 kDa; Observed MW: 64 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey,Pig |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于Phospho-AMPK α1/2 (Thr183/Thr172)抗体的3篇经典参考文献摘要：
1. **"AMP-activated protein kinase: a key system mediating metabolic responses to exercise"**
- **作者**：Hardie, D.G. (2004)
- **摘要**：该综述总结了AMPK的结构和功能，强调Thr172位点的磷酸化是AMPK激活的关键步骤，并讨论了使用特异性抗体检测该磷酸化位点在代谢研究中的应用。
2. **"Complexes between the LKB1 tumor suppressor, STRADα/β and MO25α/β are upstream kinases in the AMP-activated protein kinase cascade"**
- **作者**：Hawley, S.A. et al. (2003)
- **摘要**：研究通过免疫印迹实验(使用Phospho-AMPK α Thr172抗体)证实LKB1复合物是AMPK的上游激酶，揭示了AMPK在能量应激下的磷酸化调控机制。
3. **"Calmodulin-dependent protein kinase kinase-β is an alternative upstream kinase for AMP-activated protein kinase"**
- **作者**：Woods, A. et al. (2005)
- **摘要**：利用Phospho-AMPK α Thr172抗体，研究发现CaMKKβ可绕过LKB1直接磷酸化激活AMPK，为AMPK在钙信号通路中的作用提供了实验证据。
这些文献均通过该抗体验证AMPK活性状态，适用于代谢、癌症和信号转导领域研究。
The Phospho-AMPK alpha 1/2 (Thr183/Thr172) antibody is a critical tool for studying AMP-activated protein kinase (AMPK) activation, a central regulator of cellular energy homeostasis. AMPK, a heterotrimeric enzyme composed of alpha (catalytic), beta, and gamma subunits, senses energy deficits via rising AMP/ATP ratios. Activation requires phosphorylation of the alpha subunit at specific residues: Thr183 in alpha 1 (human isoform) and Thr172 in alpha 2. These phosphorylation events, mediated by upstream kinases like LKB1 or CaMKKβ, trigger conformational changes that enhance AMPK activity, enabling its role in restoring energy balance by promoting catabolic pathways (e.g., glucose uptake, fatty acid oxidation) and inhibiting anabolic processes (e.g., lipid synthesis).
This antibody specifically detects phosphorylated Thr183/Thr172 residues, serving as a marker for AMPK activation status. It is widely used in research to investigate metabolic disorders (e.g., diabetes, obesity), cancer (where AMPK modulates cell proliferation), and aging. Validations often include Western blotting, immunohistochemistry, or immunoprecipitation in models with metabolic stressors (e.g., exercise, nutrient deprivation) or pharmacological AMPK activators (e.g., AICAR, metformin). Cross-reactivity between alpha 1 and alpha 2 isoforms depends on sequence homology, requiring validation in specific experimental systems. Proper controls (e.g., unphosphorylated lysates, phosphatase treatment) ensure specificity. Understanding AMPK phosphorylation dynamics via this antibody provides insights into cellular adaptation to stress and therapeutic targeting of metabolic diseases.
×
