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Rabbit Polyclonal Phospho-PKARIIalpha(Ser99) Antibody

  • 中文名： Phospho-PKA RII alpha (Ser99)抗体
  • 别    名： PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit
货号: IPDX20349
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat,Pig
IF 1/20 Human,Mouse,Rat,Pig
IHC 1/50-1/100 Human,Mouse,Rat,Pig
ICC 1/50-1/200 Human,Mouse,Rat,Pig
FCM 咨询技术 Human,Mouse,Rat,Pig
Elisa 咨询技术 Human,Mouse,Rat,Pig

产品详情

AliasesPRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit
Entrez GeneID5576
WB Predicted band sizeCalculated MW: 46 kDa; Observed MW: 51 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Pig
ImmunogenA synthesized peptide derived from human Phospho-PKA R2 (S99)
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3篇与Phospho-PKA RIIα (Ser99)抗体相关的参考文献(均为虚构示例，供格式参考)：

1. **文献名称**: *PKA RIIα Ser99 phosphorylation regulates cAMP-dependent compartmentalization*

**作者**: Smith J et al.

**摘要**: 本研究揭示了Ser99磷酸化通过调控PKA RIIα亚基与AKAP蛋白的结合，影响cAMP信号通路的亚细胞定位，并在心肌细胞中验证了该抗体的特异性应用。

2. **文献名称**: *Dynamic phosphorylation of PKA regulatory subunits in neuronal plasticity*

**作者**: Chen L & Wang H

**摘要**: 文章利用Phospho-PKA RIIα (Ser99)抗体，发现海马神经元中该位点的磷酸化水平与长时程增强(LTP)相关，提示其在突触可塑性中的调控作用。

3. **文献名称**: *A novel crosstalk between PKA and mTOR signaling via RIIα phosphorylation*

**作者**: Kim S et al.

**摘要**: 研究通过免疫沉淀和Western blot(使用Ser99特异性抗体)，证明营养胁迫下mTORC1通过磷酸化PKA RIIα Ser99位点抑制cAMP信号，促进细胞存活。

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**注意**：以上文献为模拟内容，实际文献需通过PubMed/Google Scholar检索关键词如"PKA RIIα Ser99 phosphorylation"或抗体货号(如Cell Signaling Technology #XXXX)获取。

背景信息

The Phospho-PKA RII alpha (Ser99) antibody detects the phosphorylated form of the regulatory subunit RIIα of cAMP-dependent protein kinase (PKA) at serine 99. PKA, a central kinase in cAMP signaling, consists of two regulatory (R) and two catalytic (C) subunits. The R subunits (RIα, RIβ, RIIα, RIIβ) maintain PKA in an inactive state until cAMP binding triggers dissociation and activation of C subunits. RIIα, encoded by PRKAR2A, localizes PKA to specific subcellular compartments via A-kinase anchoring proteins (AKAPs), ensuring spatial-temporal signaling precision. Phosphorylation of RIIα at Ser99 occurs through autophosphorylation or other kinases, modulating PKA holoenzyme stability, AKAP interactions, or feedback regulation. This post-translational modification is implicated in cellular responses to cAMP fluctuations, such as metabolic regulation, stress adaptation, and cell cycle progression. The Phospho-PKA RII alpha (Ser99) antibody is widely used in studies investigating cAMP/PKA pathway dynamics, particularly in contexts like cardiovascular function, neuronal signaling, and cancer, where aberrant PKA activity is linked to disease. Its specificity for the phosphorylated epitope makes it a critical tool for assessing RIIα activation status and dissecting mechanisms underlying PKA-dependent signaling networks.

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