| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PTK2B; FAK2; PYK2; RAFTK; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Pro |
| Entrez GeneID | 2185 |
| WB Predicted band size | Calculated MW: 116 kDa; Observed MW: 116 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide of human PTK2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 **Phospho-PYK2 (Tyr402)** 抗体的参考文献及简要摘要：
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1. **文献名称**: *"FAK and PYK2/CAKβ in the Nervous System: A Link between Neuronal Activity, Plasticity and Survival?"*
**作者**: Avraham H, Park SY, Schinkmann K, Avraham S
**摘要**: 研究探讨了PYK2(Tyr402磷酸化)在中枢神经系统中的功能，发现其磷酸化与神经元突触可塑性和活性依赖性信号传导相关，抗体用于检测海马神经元中Tyr402位点的激活。
2. **文献名称**: *"Role of PYK2 in the Regulation of EGFR Signaling and Cancer Cell Migration"*
**作者**: Li X, Earp HS, Park EE
**摘要**: 文章揭示了PYK2在表皮生长因子受体(EGFR)信号通路中的作用，Tyr402磷酸化抗体用于验证PYK2激活对乳腺癌细胞迁移和侵袭的调控机制。
3. **文献名称**: *"Integrin Signaling through Focal Adhesion Kinase and PYK2: Implications in Tumor Progression"*
**作者**: Sieg DJ, Ilic D, Jones JC, Schlaepfer DD
**摘要**: 分析了整合素信号中PYK2(Tyr402)的磷酸化如何与FAK协同调控肿瘤细胞的黏附和转移，抗体用于检测人胶质瘤细胞中PYK2的激活状态。
4. **文献名称**: *"Phosphorylation of PYK2 at Tyr402 regulates its interaction with NADPH oxidase in inflammatory responses"*
**作者**: Okutani M, Sasaki T
**摘要**: 研究证明巨噬细胞中Tyr402磷酸化的PYK2通过结合p47phox激活NADPH氧化酶，抗体用于验证炎症反应中活性氧(ROS)生成的分子机制。
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以上文献均通过Phospho-PYK2 (Tyr402)抗体研究了该位点在细胞信号、癌症或免疫中的功能，具体实验方法可参考原文。
**Phospho-PYK2 (Tyr402) Antibody Background**
The Phospho-PYK2 (Tyr402) antibody is a specialized tool used to detect the activated form of Proline-Rich Tyrosine Kinase 2 (PYK2. also known as PTK2B or FAK2) when phosphorylated at tyrosine residue 402. PYK2. a non-receptor tyrosine kinase belonging to the focal adhesion kinase (FAK) family, plays a critical role in cellular processes such as adhesion, migration, proliferation, and apoptosis. Its activation is tightly regulated by autophosphorylation at Tyr402. a key event that triggers downstream signaling pathways, including MAPK, PI3K/Akt, and Src-mediated cascades.
This antibody specifically recognizes the phosphorylated state of Tyr402. serving as a marker for PYK2 activation in response to stimuli like calcium influx, stress signals, or integrin engagement. Researchers employ it in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study PYK2's role in physiological and pathological contexts. Dysregulation of PYK2 activity has been linked to diseases including cancer (e.g., metastasis, invasion), neurodegenerative disorders, inflammatory conditions, and bone metabolism abnormalities.
By enabling precise detection of PYK2 activation dynamics, the Phospho-PYK2 (Tyr402) antibody aids in elucidating molecular mechanisms underlying cellular responses and disease progression, making it valuable for both basic research and therapeutic target validation.
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