| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIMK1; LIMK; LIM domain kinase 1; LIMK-1 |
| Entrez GeneID | 3984 |
| WB Predicted band size | Calculated MW: 73 kDa; Observed MW: 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide of human LIMK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
+ +
以下是关于Phospho-LIM Kinase 1 (Thr508)抗体的参考文献，按研究背景与应用领域分类整理：
1. **"Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization"**
- **作者**：Arber, S. et al.
- **摘要**：研究LIMK1在Rac信号通路中的作用，证实Thr508磷酸化是LIMK1激活的关键步骤，通过该抗体检测磷酸化状态，揭示其对cofilin的调控及肌动蛋白重塑的影响。
2. **"Rho-kinase phosphorylates LIM-kinase 1 to regulate actin dynamics during cell migration"**
- **作者**：Maekawa, M. et al.
- **摘要**：探讨Rho/ROCK通路通过磷酸化LIMK1 Thr508位点调控细胞迁移的机制，利用该抗体证明其在肿瘤侵袭中的功能，为靶向治疗提供依据。
3. **"LIMK1 activation promotes neuronal growth cone collapse via cofilin inactivation"**
- **作者**：Heredia, L. et al.
- **摘要**：分析神经元中LIMK1 Thr508磷酸化对轴突导向的影响，使用该抗体验证其在神经退行性疾病(如阿尔茨海默病)中的异常激活与tau病理的相关性。
4. **"Targeting LIM kinase 1/cofilin pathway as a therapeutic strategy for melanoma metastasis"**
- **作者**：Wang, W. et al.
- **摘要**：评估抑制LIMK1 Thr508磷酸化对黑色素瘤转移的疗效，通过该抗体检测临床样本中磷酸化水平，提出靶向此通路可降低癌细胞侵袭性。
以上文献覆盖了LIMK1磷酸化的分子机制、细胞迁移、神经病理及癌症治疗等方向，均直接或间接应用该抗体进行功能研究。
Phospho-LIM Kinase 1 (Thr508) antibodies are essential tools for studying the activation and regulatory mechanisms of LIM Kinase 1 (LIMK1), a key enzyme involved in cytoskeletal dynamics. LIMK1 phosphorylates and inactivates cofilin, an actin-depolymerizing protein, thereby promoting actin filament stabilization. Its activity is tightly regulated by phosphorylation, particularly at Thr508 within its activation loop. This phosphorylation, mediated by upstream kinases such as ROCK (Rho-associated kinase) or PAK (p21-activated kinase), enhances LIMK1's catalytic activity, enabling it to modulate cellular processes like cell migration, division, and synaptic plasticity.
Antibodies targeting phosphorylated Thr508 (p-LIMK1 Thr508) are widely used to detect LIMK1 activation status in various experimental settings, including Western blotting, immunofluorescence, and immunohistochemistry. Researchers employ these antibodies to investigate signaling pathways in diseases where LIMK1 dysregulation is implicated, such as cancer metastasis, neurological disorders (e.g., Alzheimer’s disease), and vascular remodeling. For example, elevated p-LIMK1 levels correlate with invasive tumor behavior or amyloid-beta-induced neuronal toxicity.
These antibodies often undergo validation in specific cell types or tissues to confirm specificity, particularly since cross-reactivity with related kinases (e.g., LIMK2) or nonspecific phospho-epitopes can occur. Proper controls, such as phosphorylation-deficient mutants or kinase inhibition assays, are critical for accurate interpretation. Overall, p-LIMK1 (Thr508) antibodies provide critical insights into Rho GTPase-mediated signaling and its pathological consequences.
×
