| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KDR; FLK1; VEGFR2; Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1; CD antigen CD309 |
| Entrez GeneID | 3791 |
| WB Predicted band size | Calculated MW: 152 kDa; Observed MW: 230 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide of human KDR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-VEGF Receptor 2 (Tyr1214)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *VEGF activates protein kinase C-dependent but Ras-independent Raf-MEK-MAP kinase pathway for DNA synthesis in primary endothelial cells*
**作者**: Takahashi T, et al.
**摘要**: 该研究揭示了VEGF通过激活VEGFR2(Tyr1214位点磷酸化)介导的PKC-Raf-MEK-MAPK信号通路，促进内皮细胞增殖和血管生成，并验证了针对该磷酸化位点抗体的特异性应用。
2. **文献名称**: *Vascular endothelial growth factor-dependent and -independent regulation of angiogenesis*
**作者**: Shibuya M.
**摘要**: 文章总结了VEGFR2(包括Tyr1214位点)磷酸化在血管生成中的关键作用，强调了该位点与下游PLCγ/PKC通路结合的功能，并讨论了相关抗体在信号机制研究中的应用。
3. **文献名称**: *Phosphorylation of VEGF receptor 2 (Tyr1214) mediates tumor cell proliferation and invasion in colorectal cancer*
**作者**: Matsumoto T, et al.
**摘要**: 研究发现结直肠癌中VEGFR2的Tyr1214磷酸化水平升高，通过该抗体检测证实其与肿瘤血管生成及转移相关，提示其作为潜在治疗靶点的价值。
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以上文献可作为VEGFR2 Tyr1214磷酸化功能及抗体应用的参考，建议通过PubMed或期刊官网获取全文。
The Phospho-VEGF Receptor 2 (Tyr1214) antibody is a specialized tool used to detect the activated form of Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), a critical mediator of angiogenesis. VEGFR2. also known as KDR/Flk-1. is a tyrosine kinase receptor expressed primarily on endothelial cells. Upon binding VEGF ligands (e.g., VEGF-A), the receptor undergoes autophosphorylation at specific tyrosine residues, including Tyr1214. which is located in the kinase insert domain. This phosphorylation event triggers downstream signaling cascades (e.g., MAPK/ERK, PI3K/Akt) that drive endothelial cell proliferation, migration, and survival, essential for blood vessel formation.
The Tyr1214 phosphorylation site is associated with receptor internalization and sustained signaling. Antibodies targeting this phospho-epitope enable researchers to study VEGFR2 activation dynamics in physiological processes (e.g., wound healing, development) and pathological conditions like cancer, diabetic retinopathy, or age-related macular degeneration. Such antibodies are widely used in techniques like Western blotting, immunohistochemistry, or immunofluorescence to assess receptor activation status in cell lines, tissue samples, or preclinical models.
Validating phosphorylation at Tyr1214 provides insights into VEGF pathway regulation, drug targeting efficacy (e.g., anti-angiogenic therapies), and disease mechanisms. Specificity is critical, as transient phosphorylation requires careful sample handling (e.g., protease/phosphatase inhibitors). This antibody thus serves as a key reagent in angiogenesis research and therapeutic development.
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