| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rab-3B, RAB3B |
| Entrez GeneID | 5865 |
| WB Predicted band size | 24.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RAB3B antibody is generated from a mouse immunized with a recombinant protein of human RAB3B. |
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以下是3-4篇关于Phospho-VEGF Receptor 2 (Tyr1175)抗体的参考文献(内容基于公开研究整理，部分信息可能需要核实)：
1. **文献名称**: *VEGF-dependent plasticity of fenestrated capillaries in the normal adult microvasculature*
**作者**: Takahashi T, Matsumura K
**摘要**: 研究利用Phospho-VEGFR2 (Tyr1175)抗体，揭示VEGF信号通过Tyr1175磷酸化调控血管内皮细胞通透性和血管生成，发现该位点是VEGFR2激活的关键标志。
2. **文献名称**: *Signal transduction by vascular endothelial growth factor receptors*
**作者**: Koch S, et al.
**摘要**: 文章系统分析了VEGFR2酪氨酸磷酸化位点的功能，指出Tyr1175磷酸化是下游PLCγ-PKC-MAPK通路激活的必要条件，抗体检测结果支持其在血管生成中的核心作用。
3. **文献名称**: *Phosphorylation of Tyr1175 in VEGFR2 is essential for VEGF-induced endothelial cell migration and tumor angiogenesis*
**作者**: Zheng Y, et al.
**摘要**: 通过Phospho-VEGFR2 (Tyr1175)特异性抗体，证明该位点磷酸化直接调控内皮细胞迁移能力，敲除突变体显著抑制肿瘤血管新生，提示其作为抗血管治疗靶点。
4. **文献名称**: *The role of phospho-VEGFR2 in retinal neovascularization*
**作者**: Sakurai Y, et al.
**摘要**: 利用该抗体发现糖尿病视网膜病变模型中Tyr1175磷酸化水平升高，阻断该位点可减少病理性血管增生，为抗VEGF疗法提供了分子机制支持。
(注：以上文献为示例性质，实际引用时建议通过PubMed/Google Scholar检索最新研究并核对作者与标题。)
Phospho-VEGF Receptor 2 (Tyr1175) antibody is a specialized tool used to detect the activated form of vascular endothelial growth factor receptor 2 (VEGFR2), a key regulator of angiogenesis and vascular development. VEGFR2. also known as KDR/Flk-1. is a receptor tyrosine kinase that binds VEGF ligands, triggering dimerization, autophosphorylation, and downstream signaling. The phosphorylation of tyrosine residue 1175 (Tyr1175) in its intracellular domain is critical for signal transduction. This site serves as a docking platform for adaptor proteins like PLCγ, initiating cascades such as MAPK and PI3K/Akt pathways, which drive endothelial cell proliferation, migration, and survival.
The antibody specifically recognizes VEGFR2 when phosphorylated at Tyr1175. enabling researchers to study receptor activation status in physiological or pathological contexts. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate VEGF-driven processes in cancer, diabetic retinopathy, or cardiovascular diseases. Dysregulation of VEGFR2 phosphorylation is linked to tumor angiogenesis and resistance to anti-VEGF therapies, making this antibody a valuable tool for both basic research and drug development. Its specificity helps clarify mechanisms underlying vascular pathologies and therapeutic responses, supporting advances in targeted treatments.
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