| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Entrez GeneID | 5864 |
| clone | 6D1A12 |
| WB Predicted band size | 25kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Rab3a (AA: 1-220) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于Phospho-PTEN (Ser380)抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*Phosphorylation of the PTEN tail acts as an inhibitory switch by preventing its recruitment into a protein complex*
**作者**：Vazquez, F. et al.
**摘要**：该研究揭示了PTEN C端(包括Ser380位点)的磷酸化通过阻碍其与膜结合蛋白的相互作用，抑制PTEN的肿瘤抑制功能。研究使用Phospho-PTEN (Ser380)抗体验证了磷酸化状态对PTEN构象及功能的影响。
2. **文献名称**：*Regulation of PTEN stability and signaling by phosphorylation of Ser380*
**作者**：Torres, J. & Pulido, R.
**摘要**：文章阐明了Ser380磷酸化通过稳定PTEN的C端结构，减少泛素化介导的降解，从而增强其蛋白稳定性。实验中利用Phospho-PTEN (Ser380)抗体进行Western blot分析，证实了磷酸化水平与PTEN半衰期的关联。
3. **文献名称**：*CK2 and GSK3-dependent phosphorylation of PTEN regulates its localization and function in cells*
**作者**：Odriozola, L. et al.
**摘要**：研究证明CK2激酶介导的Ser380磷酸化促进了PTEN的核质穿梭，并影响其脂质磷酸酶活性。通过Phospho-PTEN (Ser380)抗体的免疫荧光实验，观察到磷酸化PTEN在细胞核内的定位变化。
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这些文献均聚焦于PTEN在Ser380位点的磷酸化对其功能、稳定性及亚细胞定位的调控机制，并明确使用了特异性抗体进行实验验证。
The Phospho-PTEN (Ser380) antibody is a critical tool for studying post-translational modifications of the tumor suppressor protein PTEN (Phosphatase and Tensin Homolog). PTEN acts as a lipid phosphatase that counteracts the PI3K/AKT/mTOR signaling pathway by dephosphorylating phosphatidylinositol (3.4.5)-trisphosphate (PIP3), thereby regulating cell growth, survival, and metabolism. Its activity and stability are tightly regulated by phosphorylation, particularly within its C-terminal regulatory domain. Serine 380 (Ser380) is a key phosphorylation site in this region, along with neighboring residues (e.g., Ser382. Thr383). Phosphorylation at Ser380. mediated by kinases such as CK2 (Casein Kinase 2), induces a conformational change that stabilizes PTEN but reduces its membrane affinity and phosphatase activity. This modification creates a "closed" state, limiting PTEN’s tumor-suppressive function while protecting it from proteasomal degradation.
The Phospho-PTEN (Ser380) antibody specifically detects PTEN phosphorylated at Ser380. enabling researchers to assess PTEN regulation in cellular contexts. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study PTEN inactivation mechanisms in cancer, neurodegeneration, and metabolic disorders. Dysregulated PTEN phosphorylation is linked to tumor progression, as hyperphosphorylation may impair its tumor-suppressive role. This antibody helps elucidate how PTEN activity is modulated by signaling pathways and informs therapeutic strategies targeting PTEN-related pathologies. Validation in specific experimental models is essential, as phosphorylation patterns may vary across cell types or disease states.
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