| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIP10 |
| Uniprot No | Q15642 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-601aa |
| 氨基酸序列 | MDWGTELWDQ FEVLERHTQW GLDLLDRYVK FVKERTEVEQ AYAKQLRSLV KKYLPKRPAK DDPESKFSQQ QSFVQILQEV NDFAGQRELV AENLSVRVCL ELTKYSQEMK QERKMHFQEG RRAQQQLENG FKQLENSKRK FERDCREAEK AAQTAERLDQ DINATKADVE KAKQQAHLRS HMAEESKNEY AAQLQRFNRD QAHFYFSQMP QIFDKLQDMD ERRATRLGAG YGLLSEAELE VVPIIAKCLE GMKVAANAVD PKNDSHVLIE LHKSGFARPG DVEFEDFSQP MNRAPSDSSL GTPSDGRPEL RGPGRSRTKR WPFGKKNKPR PPPLSPLGGP VPSALPNGPP SPRSGRDPLA ILSEISKSVK PRLASFRSLR GSRGTVVTED FSHLPPEQQR KRLQQQLEER SRELQKEVDQ REALKKMKDV YEKTPQMGDP ASLEPQIAET LSNIERLKLE VQKYEAWLAE AESRVLSNRG DSLSRHARPP DPPASAPPDS SSNSASQDTK ESSEEPPSEE SQDTPIYTEF DEDFEEEPTS PIGHCVAIYH FEGSSEGTIS MAEGEDLSLM EEDKGDGWTR VRRKEGGEGY VPTSYLRVTL N |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIP10重组蛋白的3篇参考文献概览(注：文献标题与作者为虚构示例，仅供格式参考)：
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1. **文献名称**: *"TRIP10 regulates endocytic trafficking through its interaction with the Cdc42 GTPase"*
**作者**: Smith A, et al.
**摘要**: 本研究利用重组TRIP10蛋白进行体外结合实验，发现其通过C端结构域特异性结合Cdc42.调控网格蛋白介导的内吞作用，揭示了TRIP10在细胞膜运输中的分子机制。
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2. **文献名称**: *"Structural insights into the oligomerization of TRIP10 in cytoskeletal remodeling"*
**作者**: Chen L, et al.
**摘要**: 通过表达纯化重组人源TRIP10蛋白并结合冷冻电镜分析，发现其可形成二聚体并通过特定结构域招募下游效应蛋白(如WAVE复合物)，从而促进肌动蛋白重塑和细胞迁移。
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3. **文献名称**: *"TRIP10 interacts with APPL1 to modulate EGFR signaling in cancer progression"*
**作者**: Wang Y, et al.
**摘要**: 研究利用重组TRIP10蛋白进行免疫共沉淀实验，证实其与衔接蛋白APPL1的相互作用，并证明该互作通过增强EGFR信号通路促进乳腺癌细胞的侵袭性生长。
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**备注**：实际文献需通过PubMed或Google Scholar检索关键词(如“TRIP10 recombinant”“TRIP10 signaling”)，建议结合具体研究方向筛选近年高影响力论文。
TRIP10 (Thyroid Receptor Interacting Protein 10), also known as Cdc42-interacting protein 4 (CIP4), is a multifunctional adaptor protein involved in regulating cytoskeletal dynamics, membrane trafficking, and cellular signaling. It belongs to the F-BAR (FCH-BAR) protein family, characterized by an N-terminal FCH domain and a C-terminal SH3 domain, which mediate membrane curvature sensing and protein-protein interactions, respectively. TRIP10 plays a key role in coordinating actin remodeling and endocytic processes by linking Cdc42/Rho GTPase signaling to downstream effectors like N-WASP and dynamin. It is implicated in clathrin-mediated endocytosis, vesicle transport, and cell migration.
Recombinant TRIP10 proteins are engineered to study its structure-function relationships, interaction networks, and regulatory mechanisms. These proteins are typically expressed in bacterial or mammalian systems with tags (e.g., His, GST) for purification and detection. Researchers utilize recombinant TRIP10 to dissect its binding affinities with partners such as Cdc42. TC10. and membrane phospholipids, as well as to explore its role in pathological conditions. Dysregulation of TRIP10 has been associated with cancer metastasis, neurological disorders, and immune dysfunctions, making it a potential therapeutic target. Its recombinant forms enable in vitro assays, structural studies (e.g., crystallography), and drug screening platforms aimed at modulating TRIP10-dependent pathways.
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