| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FGFR4; JTK2; TKF; Fibroblast growth factor receptor 4; FGFR-4; CD334 |
| Entrez GeneID | 2264 |
| WB Predicted band size | Calculated MW: 88 kDa; Observed MW: 88 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human FGFR4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-FGFR4 (Tyr642)抗体的参考文献示例(文献为假设性示例，实际引用需核实)：
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1. **文献名称**: "FGFR4 phosphorylation at Tyr642 regulates hepatocellular carcinoma progression via MAPK signaling"
**作者**: Wang et al.
**摘要**: 研究利用Phospho-FGFR4 (Tyr642)抗体，发现肝癌细胞中Tyr642位点的磷酸化激活MAPK通路，促进肿瘤细胞增殖和侵袭。
2. **文献名称**: "Role of FGFR4 tyrosine 642 phosphorylation in skeletal muscle differentiation"
**作者**: Martinez et al.
**摘要**: 通过Western blot和免疫荧光分析，揭示Tyr642磷酸化在成肌细胞分化中调控FGFR4与下游蛋白的相互作用，影响肌肉生成。
3. **文献名称**: "Targeting phospho-FGFR4 (Y642) overcomes drug resistance in rhabdomyosarcoma"
**作者**: Chen et al.
**摘要**: 使用Phospho-FGFR4 (Tyr642)特异性抗体证实，抑制该位点磷酸化可逆转横纹肌肉瘤对化疗的耐药性，为靶向治疗提供依据。
4. **文献名称**: "Structural basis of FGFR4 activation by phosphorylation at Tyr642"
**作者**: Kumar et al.
**摘要**: 结合晶体学和抗体检测技术，阐明Tyr642磷酸化诱导FGFR4构象变化，促进受体二聚化及信号转导的分子机制。
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**注意**：以上文献为示例，实际研究中需通过PubMed或Google Scholar等平台以关键词“Phospho-FGFR4 Tyr642”或“FGFR4 Y642 phosphorylation”检索真实文献。
The Phospho-FGFR4 (Tyr642) antibody is a specialized tool used to detect the activated form of Fibroblast Growth Factor Receptor 4 (FGFR4) when phosphorylated at tyrosine residue 642. FGFR4. a member of the receptor tyrosine kinase (RTK) family, plays critical roles in regulating cell proliferation, differentiation, migration, and survival by binding FGF ligands and activating downstream signaling pathways, including MAPK/ERK, PI3K/AKT, and STAT. Phosphorylation at Tyr642 is a key event in FGFR4 activation, typically induced by ligand binding, dimerization, and autophosphorylation. This modification triggers conformational changes that facilitate the recruitment of adaptor proteins and signaling molecules.
The Phospho-FGFR4 (Tyr642) antibody is widely utilized in research to study FGFR4 signaling dynamics in physiological processes (e.g., tissue repair, metabolism) and pathological conditions, particularly cancers such as hepatocellular carcinoma, breast cancer, and rhabdomyosarcoma, where aberrant FGFR4 activation is linked to tumor progression and drug resistance. It is commonly employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess receptor activation status in cell lines, tissues, or preclinical models. Researchers also use this antibody to evaluate the efficacy of FGFR4-targeted therapies or investigate mechanisms underlying FGFR4-related diseases. Specificity validation via knockout/knockdown controls or peptide competition is recommended to ensure accurate detection.
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