| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclic AMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67 |
| Entrez GeneID | 468 |
| WB Predicted band size | Calculated MW: 39 kDa; Observed MW: 39 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ATF4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是模拟生成的关于 **Phospho-ATF4 (Ser219)** 抗体的参考文献示例(仅供学术参考，非真实文献)：
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1. **文献名称**: *"Phosphorylation of ATF4 at Ser219 mediates its nuclear translocation during ER stress"*
**作者**: Zhang, L. et al.
**摘要**: 研究揭示了内质网应激(ER stress)下，ATF4在Ser219位点的磷酸化通过促进其核转位激活下游靶基因(如CHOP)，使用Phospho-ATF4 (Ser219)抗体验证了磷酸化事件与应激反应的关联。
2. **文献名称**: *"A PERK-dependent phosphorylation switch on ATF4 controls proteostasis"*
**作者**: Harding, H.P. et al.
**摘要**: 发现PERK激酶通过磷酸化ATF4的Ser219位点调控其稳定性，利用特异性抗体证明该修饰在蛋白质稳态失衡(如肿瘤微环境)中的关键作用。
3. **文献名称**: *"Phospho-ATF4 (Ser219) as a biomarker for oxidative stress in neuronal cells"*
**作者**: Chen, Y. et al.
**摘要**: 开发了一种基于Phospho-ATF4 (Ser219)抗体的检测方法，证明该磷酸化标记在神经元氧化应激模型中显著上调，提示其作为神经退行性疾病的潜在标志物。
4. **文献名称**: *"Site-specific phosphorylation of ATF4 coordinates metabolic adaptation in cancer"*
**作者**: Wang, Q. et al.
**摘要**: 通过CRISPR筛选和Phospho-ATF4 (Ser219)抗体验证，揭示了肿瘤细胞中ATF4磷酸化通过调控谷氨酰胺代谢促进生存的机制。
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如需真实文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词“ATF4 Ser219 phosphorylation antibody”或联系抗体供应商(如CST、Abcam)获取相关技术文献。
The Phospho-ATF4 (Ser219) antibody is a specialized tool used to detect the activated form of Activating Transcription Factor 4 (ATF4), a key regulator of cellular stress responses. ATF4 is a basic leucine zipper (bZIP) transcription factor that integrates signals from various stress pathways, including the integrated stress response (ISR), endoplasmic reticulum (ER) stress, and amino acid deprivation. Its phosphorylation at serine 219 (Ser219) is critical for modulating its transcriptional activity, stability, or interaction with co-regulators. This post-translational modification often occurs downstream of kinases such as PERK (PKR-like ER kinase) or RSK (ribosomal S6 kinase), linking cellular stress signals to adaptive gene expression programs.
The Phospho-ATF4 (Ser219) antibody specifically recognizes this phosphorylated epitope, enabling researchers to study ATF4 activation dynamics in conditions like oxidative stress, nutrient deprivation, or ER dysfunction. It is widely used in Western blotting, immunofluorescence, and immunoprecipitation to investigate ATF4's role in processes such as autophagy, apoptosis, and metabolic adaptation. Dysregulation of ATF4 phosphorylation has been implicated in cancer, neurodegenerative diseases, and metabolic disorders, making this antibody valuable for both mechanistic studies and translational research. Validation typically includes testing in knockout models or phosphorylation-deficient mutants to ensure specificity.
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