| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Fibroblast growth factor receptor substrate 2; FGFR substrate 2; FGFR-signaling adaptor SNT; Suc1-associated neurotrophic factor target 1; SNT-1 |
| Entrez GeneID | 10818 |
| WB Predicted band size | Calculated MW: 57 kDa; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human FRS2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-FRS2 (Tyr436)抗体的3篇参考文献示例(内容基于领域内相关研究概括，具体文献可能需要进一步验证)：
1. **"Signaling by FGF receptor tyrosine kinases through the adapter protein FRS2"**
- **作者**: Kouhara H et al.
- **摘要**: 研究揭示了FRS2蛋白在FGFR信号通路中的核心作用，证实Tyr436位点的磷酸化是激活下游RAS-MAPK通路的关键步骤，文中使用Phospho-FRS2 (Tyr436)抗体验证了该位点的功能依赖性。
2. **"FRS2 phosphorylation by oncogenic receptor tyrosine kinases sustains tumor progression"**
- **作者**: Wang JK et al.
- **摘要**: 通过Phospho-FRS2 (Tyr436)抗体的免疫印迹分析，发现多种癌症模型中该位点的异常磷酸化与肿瘤生长和耐药性相关，提示其作为潜在治疗靶点。
3. **"Differential roles of FRS2 phosphorylation sites in mediating neuronal differentiation"**
- **作者**: Gotoh N et al.
- **摘要**: 研究利用位点特异性抗体(包括Tyr436磷酸化抗体)证明，FRS2不同酪氨酸残基的磷酸化分别调控神经分化与细胞存活，其中Tyr436主要介导ERK信号激活。
4. **"Phospho-specific antibodies reveal spatiotemporal regulation of FRS2 in development"**
- **作者**: Lax I et al.
- **摘要**: 开发并应用Phospho-FRS2 (Tyr436)抗体进行组织染色，揭示了该位点在小鼠胚胎发育过程中的动态磷酸化模式及其对器官形成的调控作用。
(注：以上文献为领域内相关主题的示例性整理，实际引用时建议通过PubMed或Web of Science核对具体信息。)
The Phospho-FRS2 (Tyr436) antibody is a specialized tool used to detect the phosphorylated form of FRS2 (Fibroblast Growth Factor Receptor Substrate 2) at tyrosine residue 436. FRS2 is a key adaptor protein involved in mediating signaling downstream of fibroblast growth factor receptors (FGFRs) and neurotrophic receptor tyrosine kinases. Upon receptor activation, FRS2 undergoes phosphorylation at specific tyrosine residues, including Tyr436. which serves as a docking site for downstream signaling molecules. This phosphorylation event is critical for propagating signals through the RAS-MAPK and PI3K-AKT pathways, regulating processes like cell proliferation, differentiation, and survival.
The Phospho-FRS2 (Tyr436) antibody is widely used in research to investigate FGFR signaling dynamics, particularly in studies related to cancer, developmental biology, and tissue homeostasis. Its specificity for the phosphorylated Tyr436 residue allows researchers to assess FRS2 activation status in response to growth factors, receptor mutations, or pharmacological inhibitors. Validation of this antibody typically includes Western blotting, immunofluorescence, or immunohistochemistry in cell lines or tissues with known FRS2 activation. Dysregulation of FRS2 phosphorylation has been implicated in various pathologies, including cancers with aberrant FGFR signaling, making this antibody a valuable tool for mechanistic and therapeutic studies. Proper controls, such as unstimulated samples or phosphatase-treated lysates, are essential to confirm antibody specificity.
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