| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | OSR1 |
| Entrez GeneID | 9943 |
| WB Predicted band size | Calculated MW: 58 kDa; Observed MW: 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human OXSR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-OXSR1 (Thr185)抗体的模拟参考文献示例(内容为虚构，仅供格式参考)：
1. **"OXSR1 phosphorylation at Thr185 regulates ion transport in response to osmotic stress"**
- **作者**: Fukushima A., et al.
- **摘要**: 研究通过Phospho-OXSR1 (Thr185)抗体发现，Thr185磷酸化是OXSR1激酶激活的关键步骤，调控下游SLC12A离子转运蛋白活性，参与细胞渗透压应激响应。
2. **"AMPK-mediated phosphorylation of OXSR1 at Thr185 enhances cellular antioxidant defense"**
- **作者**: Lee S.J., et al.
- **摘要**: 利用Phospho-OXSR1 (Thr185)特异性抗体，证实AMPK在氧化应激下磷酸化OXSR1 Thr185位点，增强其激酶活性并促进抗氧化基因表达。
3. **"Dynamic phosphorylation of OXSR1 Thr185 during mitochondrial stress signaling"**
- **作者**: Díaz R., et al.
- **摘要**: 通过Western blot和免疫荧光(基于Phospho-OXSR1 Thr185抗体)，揭示了线粒体应激中Thr185磷酸化的动态变化及其与凋亡通路的关联。
4. **"Structural basis of OXSR1 activation by Thr185 phosphorylation"**
- **作者**: Chen L., et al.
- **摘要**: 结合晶体学与Phospho-OXSR1 (Thr185)抗体功能实验，阐明Thr185磷酸化诱导OXSR1构象变化，促进底物结合域暴露的分子机制。
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**注意**：以上文献为示例性质，实际研究中建议通过PubMed或Google Scholar以关键词“Phospho-OXSR1 Thr185”或“OXSR1 phosphorylation”检索真实文献。
The Phospho-OXSR1 (Thr185) antibody is a specialized tool used to detect the phosphorylated form of OXSR1 (Oxidative Stress Response 1) at threonine 185. a critical post-translational modification linked to its activation. OXSR1. a serine/threonine kinase belonging to the STE20 family, plays a pivotal role in cellular responses to oxidative stress and osmotic pressure. It regulates ion transport and cell volume by phosphorylating downstream targets like the Na⁺/K⁺/2Cl⁻ cotransporter NKCC1. Phosphorylation at Thr185 is essential for OXSR1 kinase activity, triggered by stress-activated signals such as p38 MAPK pathway activation. This modification induces conformational changes, enabling OXSR1 to interact with and phosphorylate its substrates, thereby maintaining cellular homeostasis under stress conditions.
The Phospho-OXSR1 (Thr185) antibody is widely used in research to study OXSR1 activation dynamics in models of oxidative stress, inflammation, and metabolic disorders. It aids in elucidating mechanisms underlying diseases like hypertension, cancer, and neurodegenerative conditions, where dysregulated stress signaling is implicated. Validated in techniques like Western blotting, immunofluorescence, and immunoprecipitation, this antibody ensures specificity for the phosphorylated epitope, distinguishing active OXSR1 from its inactive form. Its application enhances understanding of stress-responsive pathways and potential therapeutic targets, making it a valuable tool in molecular and cellular biology research.
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