| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I |
| Entrez GeneID | 1978 |
| WB Predicted band size | Calculated MW: 13 kDa; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide of human EIF4EBP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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1. **"Regulation of 4E-BP1 phosphorylation by mTOR signaling in cancer"**
- **作者**: Gingras AC et al.
- **摘要**: 探讨mTORC1信号通路通过Thr37等位点磷酸化调控4E-BP1的机制，及其在癌症细胞增殖和蛋白质合成中的作用，研究使用特异性抗体验证磷酸化水平与肿瘤进展的关系。
2. **"Phosphorylation of 4E-BP1 Thr37/46 as a biomarker for mTOR inhibitor efficacy"**
- **作者**: Dowling RJ et al.
- **摘要**: 通过Thr37/46磷酸化特异性抗体分析临床样本，提出4E-BP1磷酸化状态可作为预测mTOR抑制剂治疗乳腺癌疗效的生物标志物。
3. **"Role of 4E-BP1 Thr37 phosphorylation in neuronal development"**
- **作者**: Banko JL et al.
- **摘要**: 利用免疫印迹和免疫荧光技术，揭示神经元分化过程中mTORC1通过Thr37磷酸化调控4E-BP1.影响突触可塑性和认知功能的分子机制。
4. **"Diet-induced obesity alters 4E-BP1 phosphorylation at Thr37/46 in adipose tissue"**
- **作者**: Le Bacquer O et al.
- **摘要**: 研究高脂饮食小鼠模型中，脂肪组织mTOR信号异常激活导致4E-BP1 Thr37/46磷酸化水平升高，特异性抗体检测显示其与胰岛素抵抗的相关性。
(注：以上文献为示例，实际引用需根据具体文章调整。)
The Phospho-4E-BP1 (Thr37) antibody is a critical tool for studying the regulation of protein synthesis and cellular growth through the mTOR (mammalian target of rapamycin) signaling pathway. 4E-BP1 (eukaryotic translation initiation factor 4E-binding protein 1) is a downstream effector of mTOR complex 1 (mTORC1). When unphosphorylated, 4E-BP1 binds to eIF4E, a cap-binding protein essential for initiating mRNA translation, thereby inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 at specific residues, including Thr37. Thr46. Ser65. and Thr70. disrupts its interaction with eIF4E, enabling translation machinery assembly. The Thr37 site is among the earliest phosphorylation events in this hierarchical process, mediated by mTORC1 activity.
This antibody specifically detects 4E-BP1 phosphorylated at Thr37. serving as a marker for mTORC1 activation. Researchers use it to investigate cellular responses to growth factors, nutrient availability, stress, or pharmacological inhibitors (e.g., rapamycin) in contexts like cancer, metabolic disorders, and aging. It is widely applied in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to assess signaling dynamics in cell lines, tissues, or preclinical models. Dysregulation of 4E-BP1 phosphorylation is linked to diseases characterized by uncontrolled proliferation, making this antibody valuable for both mechanistic studies and drug development targeting mTOR pathways.
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