| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HM3; PBS; EGBRS |
| Entrez GeneID | 1131 |
| clone | 4A6B7 |
| WB Predicted band size | 66kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CHRM3 (AA: extra mix) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于 **Phospho-Rac1 (Ser71)** 抗体的3篇参考文献及其摘要内容：
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1. **文献名称**: *"Regulation of Rho GTPase activity at the leading edge of migrating cells by p21-activated kinase"*
**作者**: Dvorsky, R. et al.
**摘要**: 研究揭示了PAK1激酶通过磷酸化Rac1的Ser71位点调控其GTPase活性，影响细胞极性形成和迁移能力，抗体用于验证Rac1在细胞前沿的磷酸化状态。
2. **文献名称**: *"Phosphorylation of Rac1 at Ser71 induces a conformational shift that disrupts its interaction with RhoGDI"*
**作者**: Heo, J. et al.
**摘要**: 通过结构生物学和细胞实验证明，Rac1在Ser71位点的磷酸化会改变其构象，导致与RhoGDI的解离，从而增强Rac1的膜定位和信号传导功能，抗体用于检测磷酸化依赖性相互作用。
3. **文献名称**: *"Phosphorylated Rac1 switches on growth factor signaling in metastatic breast cancer"*
**作者**: Mack, N.A. et al.
**摘要**: 研究发现乳腺癌转移中Phospho-Rac1 (Ser71)水平显著升高，该磷酸化事件通过激活EGFR信号促进肿瘤侵袭，抗体用于临床样本中磷酸化Rac1的免疫组化分析。
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以上文献均通过Phospho-Rac1 (Ser71)抗体探究了Rac1磷酸化在细胞迁移、信号调控及癌症中的功能机制。
The Phospho-Rac1 (Ser71) antibody is a specialized tool for detecting Rac1. a small GTPase in the Rho family, when phosphorylated at serine 71. Rac1 plays a critical role in regulating cytoskeletal dynamics, cell migration, proliferation, and membrane trafficking. Its activity is tightly controlled by cycling between GTP-bound (active) and GDP-bound (inactive) states, as well as post-translational modifications. Phosphorylation at Ser71. mediated by kinases such as AKT or PKC, modulates Rac1 function by altering its interactions with effector proteins or regulatory guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). This modification has been linked to reduced Rac1 activity, impacting cellular processes like motility and invasiveness, particularly in cancer metastasis.
The Phospho-Rac1 (Ser71) antibody enables researchers to study this regulatory mechanism in physiological and pathological contexts. It is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess Rac1 activation status in response to extracellular stimuli (e.g., growth factors, chemokines) or experimental manipulations. Validation typically includes testing specificity via knockout/knockdown controls or phospho-peptide competition assays. Researchers often employ this antibody to investigate signaling pathways in cancer, immune responses, or neurological disorders, where Rac1 dysregulation contributes to disease progression. Its applications extend to drug discovery, where inhibitors targeting Rac1 or its regulators are evaluated for therapeutic potential. Proper controls, such as total Rac1 detection, are essential to distinguish phosphorylation changes from expression-level variations.
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