| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HD5; NY-CO-9 |
| Entrez GeneID | 10014 |
| WB Predicted band size | Calculated MW: 122 kDa; Observed MW: 122 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthetic peptide of human HDAC5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-HDAC5 (Ser498)抗体的3篇参考文献及其摘要：
1. **《HDAC5 phosphorylation by PKCε regulates nuclear export and cardiac myocyte maturation》**
- **作者**：Backs, J. et al.
- **摘要**：该研究揭示了PKCε介导的HDAC5在Ser498位点的磷酸化对其核质转运的调控作用，并发现这一修饰在小鼠心肌细胞成熟过程中起关键作用，影响心脏发育相关基因的表达。
2. **《Phosphorylation-dependent regulation of HDAC5 nuclear-cytoplasmic shuttling by 14-3-3 proteins in vascular smooth muscle cells》**
- **作者**：Wang, Z. et al.
- **摘要**：文章报道了HDAC5在Ser498位点的磷酸化通过结合14-3-3蛋白促进其胞质滞留，从而抑制血管平滑肌细胞的增殖和迁移，为动脉粥样硬化治疗提供了潜在靶点。
3. **《Calcium/calmodulin-dependent kinase II regulates HDAC5 nucleocytoplasmic shuttling in neuronal survival》**
- **作者**：Chawla, S. et al.
- **摘要**：研究发现CaMKII通过磷酸化HDAC5的Ser498位点，调控其核输出并促进神经元存活相关基因的转录，揭示了该磷酸化事件在神经退行性疾病中的潜在保护机制。
以上文献均涉及Phospho-HDAC5 (Ser498)抗体的应用，聚焦于不同生理/病理模型中该位点磷酸化的功能及分子机制。
Phospho-HDAC5 (Ser498) antibodies are essential tools for studying the regulation and function of histone deacetylase 5 (HDAC5), a class IIa HDAC involved in epigenetic modulation. HDAC5 primarily deacetylates histones, promoting chromatin condensation and transcriptional repression. Its activity is tightly regulated by post-translational modifications, particularly phosphorylation. The Ser498 residue, located in the nuclear regulatory domain, is a critical phosphorylation site targeted by kinases such as calcium/calmodulin-dependent kinase II (CaMKII) and protein kinase D (PKD). Phosphorylation at Ser498 triggers HDAC5 nuclear export via 14-3-3 protein binding, relieving its repressive effects on transcription factors like MEF2. which govern cellular processes such as cardiac hypertrophy, neuronal plasticity, and muscle differentiation.
These antibodies specifically recognize HDAC5 phosphorylated at Ser498. enabling researchers to assess its activation status in response to signaling pathways. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate HDAC5 dynamics in diseases such as heart failure, cancer, and neurodegenerative disorders. Validated Phospho-HDAC5 (Ser498) antibodies are critical for elucidating how kinase-HDAC5 interactions influence gene expression and cellular adaptation to stress. Proper controls, including phosphorylation-deficient mutants or kinase inhibitors, are recommended to confirm specificity in experimental models.
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