| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STAT6; Signal transducer and activator of transcription 6; IL-4 Stat |
| Entrez GeneID | 6778 |
| WB Predicted band size | Calculated MW: 94 kDa; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide of human STAT6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 **Phospho-STAT6 (Tyr641)** 抗体的参考文献及摘要概括：
1. **"IL-4 regulates Th2 cell differentiation through STAT6 phosphorylation-dependent and -independent mechanisms"**
- **作者**: Sehra, S. et al.
- **摘要**: 研究利用 Phospho-STAT6 (Tyr641) 抗体，通过 Western blot 和流式细胞术分析 IL-4 信号诱导的 STAT6 磷酸化，揭示其在 Th2 细胞分化中的关键作用，并提出不依赖磷酸化的调控途径。
2. **"STAT6 mediates IL-4-induced inhibition of IL-12 signaling in macrophages"**
- **作者**: Ohmori, Y. et al.
- **摘要**: 通过该抗体检测巨噬细胞中 STAT6 的磷酸化状态，发现 IL-4 通过 Tyr641 磷酸化抑制 IL-12 信号，调节免疫应答平衡，为炎症性疾病机制提供依据。
3. **"Phosphorylated STAT6 (Tyr641) is a biomarker of M2 macrophage activation in human lung cancer"**
- **作者**: Zhang, Y. et al.
- **摘要**: 在肺癌微环境中，使用该抗体进行免疫组化分析，证实磷酸化 STAT6 (Tyr641) 是 M2 型肿瘤相关巨噬细胞的标志物，并与患者预后相关。
4. **"STAT6 phosphorylation at Tyr641 is required for allergic airway inflammation in a murine asthma model"**
- **作者**: Kuperman, D.A. et al.
- **摘要**: 在小鼠哮喘模型中，通过该抗体检测 STAT6 磷酸化水平，证明 Tyr641 位点的激活是 IL-13 驱动的气道高反应性和黏液分泌的必要条件。
这些文献涵盖了该抗体在免疫调控、疾病模型及临床标志物研究中的应用。
The Phospho-STAT6 (Tyr641) antibody is a critical tool for studying the activation status of Signal Transducer and Activator of Transcription 6 (STAT6), a transcription factor central to immune regulation. STAT6 is phosphorylated at tyrosine 641 (Y641) in response to interleukin-4 (IL-4) and interleukin-13 (IL-13) signaling, which triggers its dimerization, nuclear translocation, and binding to target gene promoters. This post-translational modification is essential for STAT6's role in mediating Th2 cell differentiation, B cell proliferation, and M2 macrophage polarization, processes linked to allergic responses, asthma, and anti-parasitic immunity.
The antibody specifically detects STAT6 phosphorylated at Y641. enabling researchers to assess pathway activation in various experimental models, including Western blotting, immunoprecipitation, and immunohistochemistry. Its specificity is often validated using cell lysates from IL-4/IL-13-stimulated cells or peptide competition assays to confirm target recognition. Dysregulated STAT6 phosphorylation is implicated in inflammatory diseases and cancers, making this antibody valuable for both basic research and translational studies aiming to modulate STAT6-dependent pathways. Proper controls, such as unstimulated samples or phosphatase-treated lysates, are recommended to confirm signal specificity.
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