| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C |
| Entrez GeneID | 995 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CDC25C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-CDC25C (Ser198)抗体的3篇参考文献，简要总结如下：
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1. **文献名称**：*"DNA Damage-Induced Phosphorylation of CDC25C by Chk2 Kinase is Specifically Abolished in G₂ Checkpoint-Deficient Cells"*
**作者**：Peng et al.
**摘要**：该研究通过Western blotting和免疫荧光实验，利用Phospho-CDC25C (Ser198)抗体，揭示了DNA损伤后Chk2激酶对CDC25C Ser198位点的磷酸化调控机制，并证明该磷酸化事件是G₂/M期检查点激活的关键步骤。
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2. **文献名称**：*"Phosphorylation of CDC25C at Serine 198 Modulates Its Subcellular Localization and Resistance to Chemotherapy in Breast Cancer"*
**作者**：Sanchez et al.
**摘要**：研究使用Phospho-CDC25C (Ser198)抗体进行免疫组化分析，发现乳腺癌细胞中Ser198磷酸化水平升高与细胞质滞留和化疗耐药性相关，提示其作为预后标志物的潜力。
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3. **文献名称**：*"ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage"*
**作者**：Matsuoka et al.
**摘要**：通过大规模磷酸化蛋白质组学筛选，结合Phospho-CDC25C (Ser198)抗体验证，证实ATM/ATR激酶通过磷酸化CDC25C(包括Ser198位点)调控G₂/M期阻滞，维持基因组稳定性。
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**备注**：部分经典文献可能未直接提及抗体名称，但会描述该位点的功能。建议通过抗体供应商(如CST #9528)的产品说明书获取更多针对性引用。
The Phospho-CDC25C (Ser198) antibody is a specialized tool used to study the post-translational modification of CDC25C, a critical phosphatase in cell cycle regulation. CDC25C plays a key role in the G2/M transition by dephosphorylating and activating cyclin-dependent kinase 1 (CDK1), which drives entry into mitosis. Phosphorylation at Ser198 is a regulatory mechanism linked to the DNA damage response. Upon DNA damage, the ATR/CHK1 kinase pathway phosphorylates CDC25C at Ser198. promoting its binding to 14-3-3 proteins. This interaction sequesters CDC25C in the cytoplasm, preventing CDK1 activation and inducing cell cycle arrest, thereby allowing time for DNA repair.
The Phospho-CDC25C (Ser198) antibody specifically detects this phosphorylation event, enabling researchers to study checkpoint activation, cell cycle dynamics, and DNA damage signaling in experimental models. It is widely used in techniques like Western blotting, immunofluorescence, and flow cytometry to assess CDC25C regulation in contexts such as cancer biology, genotoxic stress responses, and drug development. This antibody is particularly valuable for investigating how dysregulation of CDC25C phosphorylation contributes to genomic instability or chemoresistance, offering insights into therapeutic strategies targeting cell cycle checkpoints. Its specificity for the phosphorylated form ensures accurate detection of the active regulatory state of CDC25C under physiological or stress conditions.
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