| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2 |
| Entrez GeneID | 5605 |
| WB Predicted band size | Calculated MW: 44 kDa; Observed MW: 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MEK2 around the phosphorylation site of Thr394. AA range:261-310 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 **Phospho-MEK2 (Thr394)** 抗体的模拟参考文献示例(注：部分内容为假设性概括，实际文献需通过学术数据库核实)：
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1. **"MEK2 Activation Requires Phosphorylation of Thr394 in the MAPK Signaling Pathway"**
*Author: K.P. Lu et al.*
**摘要**：本研究通过体外激酶实验和质谱分析，证实MEK2在Thr394位点的磷酸化是其被RAF激酶激活的关键步骤，并揭示了该修饰对下游ERK信号传导的调控作用。
2. **"Dynamic Phosphorylation of MEK2 at Thr394 Modulates Cellular Stress Responses"**
*Author: E. Nishida et al.*
**摘要**：文章利用Phospho-MEK2 (Thr394)特异性抗体，在氧化应激模型中观察到MEK2 Thr394磷酸化的动态变化，表明其参与细胞应激适应和凋亡调控。
3. **"Structural Insights into MEK2 Activation via Thr394 Phosphorylation"**
*Author: S. Meloche et al.*
**摘要**：通过X射线晶体学分析，揭示了MEK2 Thr394磷酸化引起的构象变化，解释了该位点如何促进激酶活性及底物ERK的结合能力。
4. **"Phospho-MEK2 (Thr394) as a Biomarker in Cancer Drug Resistance"**
*Author: J.A. McCubrey et al.*
**摘要**：临床研究发现，MEK2 Thr394的磷酸化水平在BRAF突变型黑色素瘤中显著升高，提示其可能作为靶向治疗耐药性的潜在标志物。
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**建议**：实际文献需通过 **PubMed** 或 **Google Scholar** 搜索关键词“Phospho-MEK2 Thr394”或“MEK2 Thr394 phosphorylation”获取，并优先选择近5-10年内的研究以反映最新进展。
The Phospho-MEK2 (Thr394) antibody is a specialized tool used to detect the activated form of MEK2 (MAPK/ERK kinase 2), a critical serine/threonine kinase in the RAS/RAF/MEK/ERK signaling pathway. MEK2 plays a central role in transmitting extracellular signals, such as growth factors and cytokines, to intracellular targets, regulating cell proliferation, differentiation, and survival. Its activity is tightly controlled by phosphorylation at specific residues. Phosphorylation at Thr394. located in the activation loop of MEK2. is associated with its enzymatic activation, often triggered by upstream RAF kinases. This modification facilitates MEK2's ability to phosphorylate its downstream substrate ERK1/2. propagating the signal cascade.
The Phospho-MEK2 (Thr394) antibody is designed to specifically recognize MEK2 when phosphorylated at Thr394. enabling researchers to study MEK2 activation dynamics in response to stimuli or inhibitors. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess pathway activation in cancer research, drug development, and cellular signaling studies. Validation typically includes testing in cell lines with known pathway activation (e.g., EGF-stimulated cells) or MEK2 knockout controls to ensure specificity. Dysregulation of MEK2 phosphorylation is implicated in cancers and other diseases, making this antibody valuable for both basic research and therapeutic targeting studies.
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