首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠环加氧酶2(COX-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PTGS2含量。PTGS2，又称COX-2，是前列腺素生物合成中的关键酶，参与炎症和细胞有丝分裂。其表达受特定刺激事件调控，与多种疾病如肿瘤、炎症性疾病相关。研究PTGS2机制有助于开发针对相关疾病的治疗方法。试剂盒检测范围为1.56 ng/mL-100 ng/mL，特别适用于科研场景中炎症机制研究、药物作用评估或疾病模型中的COX - 2动态监测，例如关节炎模型、神经炎症研究或肿瘤微环境分析等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ptgs2 ELISA Kit; Cox-2 ELISA Kit; Cox2 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-2 ELISA Kit; COX-2 ELISA Kit; PHS II ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; PGH synthase 2 ELISA Kit; PGHS-2 ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit
缩写：PTGS2
Uniprot No.：P35355
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-106 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-89 | |||||
回收率：
| The recovery of rat COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-98 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.3862.2852.3362.191 501.6281.5271.5781.433 250.9370.9160.9270.782 12.50.5640.5750.5700.425 6.250.3650.3760.3710.226 3.120.2400.2510.2460.101 1.560.1950.1910.1930.048 00.1450.1440.145 | ||||||
货期：3-5 working days
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