WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 1/50-1/100 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src |
Entrez GeneID | 6714 |
WB Predicted band size | Calculated MW: 60 kDa; Observed MW: 60 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthesized peptide derived from human Phospho-Src (Y419) |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Role of Src tyrosine kinase in cancer signaling pathways"**
- **作者**: Irby, R.B., Yeatman, T.J.
- **摘要**: 研究探讨了Src酪氨酸激酶在结肠癌细胞中的激活机制,使用Phospho-Src (Tyr419)抗体检测其磷酸化水平,发现其与肿瘤侵袭性和转移相关。
2. **"Activated Src signaling in human tumors: A therapeutic target"**
- **作者**: Yeatman, T.J.
- **摘要**: 通过Phospho-Src (Tyr419)抗体分析多种癌症模型,证实Src在Tyr419位点的磷酸化与肿瘤进展相关,并评估了靶向抑制剂的治疗效果。
3. **"Focal adhesion kinase regulation by Src family kinases in integrin signaling"**
- **作者**: Frame, M.C., et al.
- **摘要**: 研究整合素信号通路中Src的激活机制,利用Phospho-Src (Tyr419)抗体揭示其在粘着斑动态重塑中的作用,为细胞迁移机制提供依据。
4. **"Targeting Src in breast cancer: Inhibition of cell proliferation and invasion"**
- **作者**: Riggins, R.B., et al.
- **摘要**: 通过Phospho-Src (Tyr419)抗体检测乳腺癌细胞中Src的活性状态,结合体内实验证明抑制其磷酸化可显著降低肿瘤生长和转移潜力。
**注**:文献中涉及的Tyr419可能对应人类Src的Tyr416(因物种编号差异),具体需结合实验模型确认。
Phospho-Src (Tyr419) antibody is a crucial tool for studying the activation status of Src kinase, a non-receptor tyrosine kinase involved in diverse cellular signaling pathways. Src plays a pivotal role in regulating processes such as cell proliferation, differentiation, adhesion, and migration. Its activity is tightly controlled by phosphorylation and dephosphorylation events. The autophosphorylation site Tyr419 (corresponding to Tyr418 in rodents) in the catalytic domain is a key marker of Src activation. Phosphorylation at this site induces a conformational shift that enhances kinase activity, enabling downstream signaling through pathways like MAPK/ERK, PI3K/Akt, and FAK, which are implicated in oncogenesis, metastasis, and cytoskeletal remodeling.
This antibody specifically recognizes Src phosphorylated at Tyr419. allowing researchers to detect active Src in various experimental models. It is widely used in Western blotting, immunohistochemistry, and immunofluorescence to investigate Src activation in cancer, bone metabolism, and neurological disorders. Dysregulated Src activity, marked by elevated Tyr419 phosphorylation, is associated with tumor progression, drug resistance, and invasive phenotypes in cancers such as breast, colon, and pancreatic cancer. Additionally, it serves as a biomarker for studying Src's role in osteoclast function and neurodegenerative diseases. Researchers must validate the antibody for species specificity, as cross-reactivity with other Src-family kinases (e.g., Lyn, Fyn) may occur depending on sequence homology. Its application advances understanding of Src-driven mechanisms and therapeutic targeting in disease contexts.
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