| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TPST1 |
| Uniprot No | O60507 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-370aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH TGSMQHAMEC HHRIEERSQP VKLESTRTTV RTGLDLKANK TFAYHKDMPL IFIGGVPRSG TTLMRAMLDA HPDIRCGEET RVIPRILALK QMWSRSSKEK IRLDEAGVTD EVLDSAMQAF LLEIIVKHGE PAPYLCNKDP FALKSLTYLS RLFPNAKFLL MVRDGRASVH SMISRKVTIA GFDLNSYRDC LTKWNRAIET MYNQCMEVGY KKCMLVHYEQ LVLHPERWMR TLLKFLQIPW NHSVLHHEEM IGKAGGVSLS KVERSTDQVI KPVNVGALSK WVGKIPPDVL QDMAVIAPML AKLGYDPYAN PPNYGKPDPK IIENTRRVYK GEFQLPDFLK EKPQTEQVE |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TPST1重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**：*Expression and characterization of recombinant human tyrosylprotein sulfotransferase-1 (TPST1) in mammalian cells*
**作者**：Smith J, Doe R, Brown K
**摘要**：该研究报道了在HEK293细胞中重组表达人源TPST1的方法，通过亲和层析纯化获得高活性酶，并验证其对模型底物(如PSGL-1)的磺酸化修饰能力，揭示了TPST1底物特异性的关键结构域。
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2. **文献名称**：*Structural and functional analysis of TPST1: Insights from a bacterial expression system*
**作者**：Zhang L, Wang H, Liu Y
**摘要**：作者利用大肠杆菌系统成功表达并纯化重组TPST1蛋白，结合X射线晶体学解析其三维结构，阐明了催化活性中心的关键氨基酸残基，为开发靶向TPST1的抑制剂提供了结构基础。
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3. **文献名称**：*TPST1-mediated tyrosine sulfation regulates chemokine receptor signaling in immune cells*
**作者**：Chen M, Patel S, Garcia A
**摘要**：研究通过重组TPST1蛋白体外实验，证明其对CCR5等趋化因子受体的酪氨酸磺酸化修饰，直接影响免疫细胞迁移和信号转导，揭示了TPST1在炎症反应中的调控机制。
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**备注**：上述文献为示例，实际引用时需通过学术数据库(如PubMed、Web of Science)检索真实文献。建议搜索关键词：*TPST1 recombinant expression, tyrosylprotein sulfotransferase-1 purification, TPST1 enzymatic activity*。
**Background of TPST1 Recombinant Protein**
Tyrosylprotein sulfotransferase 1 (TPST1) is an enzyme belonging to the sulfotransferase family, which catalyzes the transfer of sulfate groups from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to tyrosine residues within substrate proteins. This post-translational modification, termed tyrosine sulfation, plays a critical role in modulating protein-protein and protein-ligand interactions, thereby influencing diverse physiological processes such as hemostasis, inflammation, receptor signaling, and viral entry. TPST1 is a membrane-bound enzyme localized to the Golgi apparatus and is ubiquitously expressed in human tissues, with high activity observed in the liver, kidneys, and immune cells.
The recombinant TPST1 protein is produced via genetic engineering techniques, often using mammalian or insect cell expression systems to ensure proper folding and enzymatic activity. Its recombinant form enables researchers to study sulfation mechanisms in vitro, screen for substrates, or investigate dysregulation linked to diseases. For instance, aberrant tyrosine sulfation has been implicated in pathological conditions, including cancer metastasis, autoimmune disorders, and viral infections (e.g., HIV-1 entry via sulfated co-receptors).
Structurally, TPST1 contains conserved sulfotransferase domains and relies on PAPS as a co-factor. Recombinant TPST1 serves as a valuable tool for drug discovery, particularly in developing inhibitors targeting sulfation-dependent pathways. Additionally, it aids in elucidating the functional significance of tyrosine sulfation in secreted proteins, such as chemokines, adhesion molecules, and coagulation factors. Continued research on TPST1 and its recombinant variants holds promise for therapeutic innovations and deeper insights into post-translational regulatory networks.
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