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产品描述:人妊娠特异性β1糖蛋白3(PSG3)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的PSG3含量。试剂盒检测范围为0.625 ng/mL-50 ng/mL,科研人员可通过该产品探究PSG3在不同妊娠阶段的表达动态,分析其在滋养细胞疾病或某些恶性肿瘤中的潜在生物学功能,也可用于评估实验动物模型中的蛋白表达水平;为生殖生物学、肿瘤微环境等基础研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:PSG3 ELISA Kit; Pregnancy-specific beta-1-glycoprotein 3 ELISA Kit; PS-beta-G-3 ELISA Kit; PSBG-3 ELISA Kit; Pregnancy-specific glycoprotein 3 ELISA Kit; Carcinoembryonic antigen SG5 ELISA Kit
缩写:PSG3
Uniprot No.:Q16557
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.625 ng/mL-50 ng/mL
灵敏度:0.78 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Tags & Cell Markers
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<15% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PSG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 95 | |||||
Range % | 90-105 | ||||||
1:2 | Average % | 87 | |||||
Range % | 85-95 | ||||||
1:4 | Average % | 92 | |||||
Range % | 88-101 | ||||||
1:8 | Average % | 95 | |||||
Range % | 89-99 |
回收率:
The recovery of human PSG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 95 | 92-99 | |||||
EDTA plasma (n=4) | 96 | 90-102 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2Average 500.3620.3550.359 18.750.4820.4970.490 9.3750.6880.6740.681 3.1251.0131.0951.054 0.6251.3271.3421.335 01.9531.9161.935 |
货期:3-5 working days
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