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产品描述：人P选择素(P-Selectin/CD62P)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SELP含量。SELP即选择素P，是细胞黏附分子选择素家族成员。其在血小板和内皮细胞表面表达，在炎症和血栓形成过程中起重要作用。研究机制主要是介导白细胞与血管内皮细胞、血小板间黏附，促进免疫细胞迁移，还参与肿瘤转移，是潜在治疗靶点。试剂盒检测范围为0.9 ng/mL-60 ng/mL，适用于基础医学研究中P选择素相关信号通路的探索、炎症性疾病模型的分子机制解析、抗血栓药物研发的疗效评估等场景，尤其适合心血管疾病、免疫性疾病或肿瘤微环境研究中的生物标志物分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Antigen CD62 ELISA Kit; CD62 ELISA Kit; CD62 antigen-like family member P ELISA Kit; CD62P ELISA Kit; GMP 140 ELISA Kit; GMP-140 ELISA Kit; GMP140 ELISA Kit; GMRP ELISA Kit; Granule membrane protein 140 ELISA Kit; Granulocyte membrane protein ELISA Kit; GRMP ELISA Kit; LECAM 3 ELISA Kit; LECAM3 ELISA Kit; Leukocyte endothelial cell adhesion molecule 3 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 3 ELISA Kit; LYAM3_HUMAN ELISA Kit; P Selectin ELISA Kit; P-selectin ELISA Kit; PADGEM ELISA Kit; Platelet activation dependent granule-external membrane protein ELISA Kit; Platelet alpha granule membrane protein ELISA Kit; PSEL ELISA Kit; Selectin P (granule membrane protein 140kDa, antigen CD62) ELISA Kit; SELP ELISA Kit
缩写：P-Selectin/CD62P
Uniprot No.：P16109
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:20 | Average % | 98 | ||||
| Range % | 92-110 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-104 | ||||
| EDTA plasma (n=4) | 96 | 87-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected602.3052.3682.3372.269301.7951.8051.8001.732151.1461.1871.1671.0997.50.6530.6890.6710.6033.750.3440.3580.3510.2831.80.2450.2640.2550.1870.90.1100.1160.1130.04500.0660.0690.068 | ||||||
货期：3-5 working days
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