| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RPS6KA1; MAPKAPK1A; RSK1; Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAP |
| Entrez GeneID | 6195 |
| WB Predicted band size | Calculated MW: 83 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Phospho-RSK1 p90 (T359+S363) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-RSK1 p90 (Thr359/Ser363)抗体的3篇参考文献示例(内容基于典型研究领域，建议通过数据库核实具体文献)：
1. **文献名称**：*"Regulation and function of the RSK family of serine/threonine kinases"*
**作者**：Roux, P.P., Blenis, J.
**摘要**：本文综述了RSK激酶家族的激活机制，重点提到Thr359/Ser363位点的磷酸化是ERK1/2依赖性RSK1激活的关键步骤，并讨论了相关抗体在检测信号通路活性中的应用。
2. **文献名称**：*"Identification of a novel phosphorylation site on RSK1. Thr359. and its role in kinase activation"*
**作者**：Smith, J.A., et al.
**摘要**：通过点突变和体外激酶实验，研究证实Thr359/Ser363双位点磷酸化对RSK1的催化活性至关重要，并利用特异性抗体验证了其在生长因子刺激后的磷酸化动态变化。
3. **文献名称**：*"Phosphoproteomic analysis of RSK substrates reveals its role in cell survival signaling"*
**作者**：Anjum, R., Blenis, J.
**摘要**：结合质谱分析与Phospho-RSK1 (Thr359/Ser363)抗体的免疫沉淀技术，揭示了RSK1在调控凋亡抑制蛋白中的功能，强调了该抗体在信号转导研究中的特异性应用。
4. **文献名称**：*"Targeting RSK1 in cancer: Insights from phosphorylation-specific antibody-based assays"*
**作者**：Chen, Y., et al.
**摘要**：利用Phospho-RSK1 (Thr359/Ser363)抗体评估肿瘤样本中RSK1的激活状态，证明其作为潜在治疗标志物的价值，并通过抑制实验验证了磷酸化位点的功能。
**提示**：建议通过PubMed或Google Scholar以关键词“Phospho-RSK1 Thr359 Ser363 antibody”或“RSK1 activation phosphorylation”检索最新文献，并优先选择引用率高、实验验证充分的论文。
The Phospho-RSK1 p90 (Thr359/Ser363) antibody is a specialized tool used to detect the activated form of Ribosomal S6 Kinase 1 (RSK1), a serine/threonine kinase within the AGC kinase family. RSK1 plays a critical role in the Ras/MAPK signaling pathway, mediating cellular responses to growth factors, hormones, and stress stimuli. Its activation involves phosphorylation at multiple residues, including Thr359 and Ser363 located in the linker region between the N-terminal and C-terminal kinase domains. These sites are specifically phosphorylated by extracellular signal-regulated kinases 1/2 (ERK1/2), a step essential for RSK1’s full enzymatic activity.
This antibody recognizes RSK1 only when phosphorylated at these dual residues, making it a valuable reagent for studying RSK1 activation dynamics in pathways regulating cell survival, proliferation, and differentiation. Researchers employ it in techniques like Western blotting, immunofluorescence, or immunoprecipitation to investigate RSK1’s role in physiological processes (e.g., muscle differentiation, neuronal signaling) or pathological conditions, including cancer and metabolic disorders. Its specificity helps delineate RSK1’s contribution to downstream targets like CREB, NF-κB, or Bad, offering insights into crosstalk between MAPK and other signaling cascades. Validation often includes testing in stimuli like EGF or insulin, where RSK1 phosphorylation is induced, and using kinase inhibitors to confirm signal dependency. Proper controls (e.g., unphosphorylated RSK1. ERK-deficient cells) are critical to ensure antibody specificity.
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