| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TPS2; tryptaseB; tryptaseC |
| WB Predicted band size | 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Phospho-Histone H3 (Thr3)抗体的3篇参考文献：
1. **"Mitotic histone H3 phosphorylation by the kinase Haspin"**
- **作者**: Dai, J., Sultan, S., Taylor, S.S., et al.
- **摘要**: 该研究揭示了Haspin激酶特异性磷酸化组蛋白H3的Thr3位点，促进Aurora B复合体定位到有丝分裂染色体的着丝粒区域，调控染色体分离和细胞周期进程。
2. **"Haspin kinase phosphorylates histone H3 at threonine 3 during mitosis"**
- **作者**: Yamagishi, Y., Honda, T., Tanno, Y., et al.
- **摘要**: 通过体外和体内实验证实Haspin激酶直接催化组蛋白H3的Thr3磷酸化，该修饰对维持有丝分裂期间Aurora B的活性及染色体正确排列至关重要。
3. **"Phosphorylation of histone H3 Thr3 is required for the maintenance of sister chromatid cohesion"**
- **作者**: Kelly, A.E., Ghenoiu, C., Xue, J.Z., et al.
- **摘要**: 研究发现Thr3磷酸化通过招募Borealin蛋白调控姐妹染色单体的黏连蛋白复合体稳定性，影响染色体分离的保真性。
4. **"Regulation of mitotic chromosome cohesion by Haspin and Aurora B kinase"**
- **作者**: Eswaran, J., Patnaik, D., Filippakopoulos, P., et al.
- **摘要**: 探讨了Haspin介导的Thr3磷酸化与Aurora B激酶的协同作用，阐明其在染色体凝集和细胞周期检查点中的分子机制。
这些文献均利用Phospho-Histone H3 (Thr3)抗体检测该修饰的生物学功能，聚焦于有丝分裂调控和表观遗传机制。
Phospho-Histone H3 (Thr3) antibodies are essential tools for studying mitotic events and chromatin dynamics during the cell cycle. Histone H3. a core component of nucleosomes, undergoes post-translational modifications, including phosphorylation, to regulate chromatin structure and gene expression. Phosphorylation at threonine 3 (Thr3) is a conserved epigenetic marker linked to chromosome condensation and segregation during mitosis. This modification is mediated by the kinase Haspin (GSG2), which specifically targets Thr3 during late G2 and early M phases. Unlike the more widely studied phosphorylation at Ser10 or Ser28. Thr3 phosphorylation facilitates the recruitment of Aurora B kinase to centromeres, a critical step for ensuring proper sister chromatid cohesion and error correction during mitosis.
Antibodies against Phospho-Histone H3 (Thr3) are widely used in cell biology to identify mitotic cells, quantify mitotic indices, and investigate defects in chromosome dynamics. They are applied in techniques like immunofluorescence, flow cytometry, and Western blotting to visualize spatial and temporal phosphorylation patterns in cells or tissues. In cancer research, these antibodies help assess tumor proliferation rates, as elevated Thr3 phosphorylation correlates with aggressive malignancies. Additionally, they serve as biomarkers for evaluating the efficacy of anti-mitotic drugs targeting Haspin or Aurora kinases. However, interpretation requires caution, as cross-reactivity with other phosphorylated residues or nonspecific binding may occur. Overall, these antibodies provide critical insights into cell cycle regulation, genomic stability, and disease mechanisms.
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