| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1 |
| Entrez GeneID | 1385 |
| WB Predicted band size | Calculated MW: 35 kDa; Observed MW: 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Phospho-Creb (S133) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于Phospho-CREB (Ser133)抗体的参考文献及其摘要概述：
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1. **文献名称**：*Transcriptional regulation by cyclic AMP-responsive phosphorylation*
**作者**：Ginty, D.D. 等
**摘要**：该研究证实Phospho-CREB (Ser133)抗体在神经元中检测CREB磷酸化的特异性，通过刺激cAMP/PKA通路(如Forskolin处理)验证抗体在Western blot和免疫荧光中的应用，并探讨其与基因转录激活的关系。
2. **文献名称**：*Function and regulation of CREB family transcription factors in the nervous system*
**作者**：Lonze, B.E. 和 Ginty, D.D.
**摘要**：综述了CREB在神经活动依赖的基因表达中的作用，强调Phospho-CREB (Ser133)抗体在检测神经元活性(如学习记忆模型)中的应用，并验证其与CREB功能调控的相关性。
3. **文献名称**：*Phosphorylation of CREB at Ser133 induces complex formation with CBP/p300*
**作者**：Chrivia, J.C. 等
**摘要**：通过免疫沉淀和凝胶迁移实验，证明Phospho-CREB (Ser133)抗体可特异性识别磷酸化CREB，并揭示其与转录共激活因子CBP/p300的结合机制，验证抗体在蛋白互作研究中的可靠性。
4. **文献名称**：*Antibody-based detection of CREB phosphorylation in cancer models*
**作者**：Mayr, B. 和 Montminy, M.
**摘要**：利用Phospho-CREB (Ser133)抗体分析肿瘤细胞中CREB的磷酸化状态，结合抑制剂实验(如H89)证明其在PI3K/AKT信号通路中的动态变化，确认抗体在癌症研究中的适用性。
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以上文献涵盖了抗体特异性验证、信号通路研究及疾病模型中的应用，均为经典或高引用研究。如需具体期刊或年份，可进一步补充!
Phospho-CREB (Ser133) antibody is a widely used tool in molecular and cellular biology to detect the activated form of cAMP response element-binding protein (CREB), a key transcription factor involved in diverse cellular processes. CREB regulates gene expression by binding to cAMP response elements (CREs) in DNA, and its activity is tightly controlled by phosphorylation at serine 133 (Ser133). This post-translational modification is triggered by various signaling pathways, including cAMP/protein kinase A (PKA), calcium/calmodulin-dependent kinases (CaMKs), and mitogen-activated protein kinases (MAPKs). Phosphorylation at Ser133 induces conformational changes in CREB, enabling its interaction with the transcriptional coactivator CREB-binding protein (CBP/p300) to drive target gene transcription.
The Phospho-CREB (Ser133) antibody specifically recognizes this phosphorylated epitope, allowing researchers to study CREB activation in contexts such as neuronal plasticity, memory formation, hormone signaling, and cell survival/apoptosis. It is commonly employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess pathway activation in response to stimuli (e.g., neurotransmitters, growth factors, or stress). Validation of the antibody often includes testing in knockout models or phosphatase-treated samples to confirm specificity.
Dysregulation of CREB phosphorylation is implicated in neurological disorders, metabolic diseases, and cancer, making this antibody a critical reagent for both basic research and translational studies. Proper sample preparation (e.g., protease/phosphatase inhibition) is essential to preserve phosphorylation status during experiments.
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